Dicer-2 and R2D2 coordinately bind siRNA to promote assembly of the siRISC complexes

Xiang Liu, Feng Jiang, Savitha Kalidas, Dean Smith, Qinghua Liu

Research output: Contribution to journalArticlepeer-review

142 Scopus citations


In Drosophila melanogaster, the Dicer-2/R2D2 complex initiates RNA interference (RNAi) by processing long double-stranded RNA (dsRNA) into small interfering RNA (siRNA). Recent biochemical studies suggest that the Dcr-2/R2D2 complex also facilitates incorporation of siRNA into the RNA-induced silencing complex (siRISC). Here we present genetic evidence that R2D2 and Dcr-2 are both required for loading siRNA onto the siRISC complex. Consistent with this, only the Dcr-2/R2D2 complex, but neither Dcr-2 nor R2D2 alone, can efficiently interact with duplex siRNA. Furthermore, both dsRNA-binding domains of R2D2 are critical for binding to siRNA and promoting assembly of the siRISC complexes. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1514-1520
Number of pages7
Issue number8
StatePublished - 2006


  • Dicer-2
  • R2D2
  • RNA interference (RNAi)
  • siRISC
  • siRNA

ASJC Scopus subject areas

  • Molecular Biology


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