Determination of secondary structure in the initiation region of ovalbumin mRNA

Charles D. Liarakos, Randolph P. Maddox, Kay A. Hilscher, Joseph R. Bishop, Darren K McGuire, Randall A. Kopper

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases In cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotldes including and following the initiation codon. A synthetic ovalbumln mRWA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.0V). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications A computer program for determining RNA secondary structure was used to find a 5′-region structure for ovalbumin mRNA that is consistent with our data.

Original languageEnglish (US)
Pages (from-to)11249-11265
Number of pages17
JournalNucleic acids research
Issue number23
StatePublished - Dec 9 1988

ASJC Scopus subject areas

  • Genetics


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