Determination of secondary structure in the initiation region of ovalbumin mRNA

Charles D. Liarakos; Randolph P. Maddox; Kay A. Hilscher; Joseph R. Bishop; Darren K McGuire; Randall A. Kopper

doi:10.1093/nar/16.23.11249

Determination of secondary structure in the initiation region of ovalbumin mRNA

Charles D. Liarakos, Randolph P. Maddox, Kay A. Hilscher, Joseph R. Bishop, Darren K McGuire, Randall A. Kopper

Research output: Contribution to journal › Article › peer-review

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Abstract

We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases In cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotldes including and following the initiation codon. A synthetic ovalbumln mRWA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.0V). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications A computer program for determining RNA secondary structure was used to find a 5′-region structure for ovalbumin mRNA that is consistent with our data.

Original language	English (US)
Pages (from-to)	11249-11265
Number of pages	17
Journal	Nucleic acids research
Volume	16
Issue number	23
DOIs	https://doi.org/10.1093/nar/16.23.11249
State	Published - Dec 9 1988

ASJC Scopus subject areas

Genetics

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@article{d9b34aa4f5564eb18a056adb85c2c8dc,

title = "Determination of secondary structure in the initiation region of ovalbumin mRNA",

abstract = "We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases In cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotldes including and following the initiation codon. A synthetic ovalbumln mRWA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.0V). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications A computer program for determining RNA secondary structure was used to find a 5′-region structure for ovalbumin mRNA that is consistent with our data.",

author = "Liarakos, {Charles D.} and Maddox, {Randolph P.} and Hilscher, {Kay A.} and Bishop, {Joseph R.} and McGuire, {Darren K} and Kopper, {Randall A.}",

note = "Funding Information: This work was supported by NSF Grant DMB 8508931. M. Zuker's PC-FOLD (Veralon 3.0) program for the determination of RNA secondary structure and J. Thompson's MOLECULE program for the 2D display of secondary structures generated by PC-FOLD were obtained from BIONET, Mountain View, CA 94040.",

year = "1988",

month = dec,

day = "9",

doi = "10.1093/nar/16.23.11249",

language = "English (US)",

volume = "16",

pages = "11249--11265",

journal = "Nucleic acids research",

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T1 - Determination of secondary structure in the initiation region of ovalbumin mRNA

AU - Liarakos, Charles D.

AU - Maddox, Randolph P.

AU - Hilscher, Kay A.

AU - Bishop, Joseph R.

AU - McGuire, Darren K

AU - Kopper, Randall A.

N1 - Funding Information: This work was supported by NSF Grant DMB 8508931. M. Zuker's PC-FOLD (Veralon 3.0) program for the determination of RNA secondary structure and J. Thompson's MOLECULE program for the 2D display of secondary structures generated by PC-FOLD were obtained from BIONET, Mountain View, CA 94040.

PY - 1988/12/9

Y1 - 1988/12/9

N2 - We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases In cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotldes including and following the initiation codon. A synthetic ovalbumln mRWA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.0V). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications A computer program for determining RNA secondary structure was used to find a 5′-region structure for ovalbumin mRNA that is consistent with our data.

AB - We have analyzed the secondary structure in the region surrounding the initiation codons of both cellular and synthetic versions of ovalbumin mRNA. RNase V1 cleavage sites and structure-dependent, chemically modified bases In cellular ovalbumin mRNA were determined by reverse transcription of hen poly A(+) RNA using ovalbumin-specific, synthetic DNA primers. These results indicate an extensive region of unpaired nucleotides preceding the initiation codon and a region of base-paired nucleotldes including and following the initiation codon. A synthetic ovalbumln mRWA (SP65.OV) was prepared by run-off transcription of a cloned ovalbumin cDNA (pSP65.0V). Identical regions of hen ovalbumin and SP65.OV mRNAs gave identical patterns of structure-dependent base modifications A computer program for determining RNA secondary structure was used to find a 5′-region structure for ovalbumin mRNA that is consistent with our data.

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JO - Nucleic acids research

JF - Nucleic acids research

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Determination of secondary structure in the initiation region of ovalbumin mRNA

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