@inbook{2642bf1a09be476fbb5247a2b815ae18,
title = "Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells",
abstract = "Dynamic pluripotent stem cell (PSC) states are in vitro adaptations of the pluripotency continuum in vivo. Previous studies have generated a number of PSCs with distinct properties. By modulating the FGF, TGF-β, and WNT pathways, we have derived intermediate PSCs (FTW-PSCs) that are permissive for direct primordial germ cell-like cell (PGC-LC) induction in vitro. Here, we describe the method for derivation and maintenance of mouse and human FTW-PSCs, as well as PGC-LC induction from FTW-PSCs.",
keywords = "Formative state, Human pluripotent stem cells, Intermediate state, Mouse pluripotent stem cells, Primordial germ cell",
author = "Leqian Yu and Emily Ballard and {Pinzon Arteaga}, {Carlos A.} and Jun Wu",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3259-8_16",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "269--280",
booktitle = "Methods in Molecular Biology",
}