Derivation and Differentiation of Human Embryonic Germ Cells

Michael J. Shamblott; Candace L. Kerr; Joyce Axelman; John W. Littlefield; Gregory O. Clark; Ethan S. Patterson; Russell C. Addis; Jennifer N. Kraszewski; Kathleen C. Kent; John D. Gearhart

doi:10.1016/B978-0-12-409503-8.00030-5

Derivation and Differentiation of Human Embryonic Germ Cells

Michael J. Shamblott, Candace L. Kerr, Joyce Axelman, John W. Littlefield, Gregory O. Clark, Ethan S. Patterson, Russell C. Addis, Jennifer N. Kraszewski, Kathleen C. Kent, John D. Gearhart

Internal Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Human embryonic germ (EG) cells can be derived from primordial germ cells (PGCs) by using methods similar to those used to derive mouse EG cultures. Like mouse embryonic stem (ES) and EG cells, human EG cells require leukemia inhibitory factor (LIF) for proliferation as undifferentiated stem cells. Unlike mouse EG cells, however, human EG cells retain dependence on exogenous cytokines and factors supplied by the feeder layer, and spontaneously differentiate into embryoid bodies (EBs) more frequently. Although EBs are not pluripotent, they express phenotypic markers found on progenitor, precursor, and mature cells. Cells retaining a capacity for cell proliferation and express makers of multiple lineages can be isolated from EBs, and can be used in a variety of in vitro and in vivo differentiation paradigms. Current challenges are to match individual EB-derived (EBD) cultures to desired endpoints, and to enrich or purify specific populations of cells within EBD cultures.

Original language	English (US)
Title of host publication	Essentials of Stem Cell Biology
Subtitle of host publication	Third Edition
Publisher	Elsevier Inc.
Pages	435-451
Number of pages	17
ISBN (Electronic)	9780124104273
ISBN (Print)	9780124095038
DOIs	https://doi.org/10.1016/B978-0-12-409503-8.00030-5
State	Published - 2014

Keywords

C-kit ligand
Culture evaluation
Differentiation markers
Embryoid bodies
Expression profiling
Human embryonic germ cells
Primordial germ cells
STO feeder layer

ASJC Scopus subject areas

Medicine(all)

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10.1016/B978-0-12-409503-8.00030-5

Cite this

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title = "Derivation and Differentiation of Human Embryonic Germ Cells",

abstract = "Human embryonic germ (EG) cells can be derived from primordial germ cells (PGCs) by using methods similar to those used to derive mouse EG cultures. Like mouse embryonic stem (ES) and EG cells, human EG cells require leukemia inhibitory factor (LIF) for proliferation as undifferentiated stem cells. Unlike mouse EG cells, however, human EG cells retain dependence on exogenous cytokines and factors supplied by the feeder layer, and spontaneously differentiate into embryoid bodies (EBs) more frequently. Although EBs are not pluripotent, they express phenotypic markers found on progenitor, precursor, and mature cells. Cells retaining a capacity for cell proliferation and express makers of multiple lineages can be isolated from EBs, and can be used in a variety of in vitro and in vivo differentiation paradigms. Current challenges are to match individual EB-derived (EBD) cultures to desired endpoints, and to enrich or purify specific populations of cells within EBD cultures.",

keywords = "C-kit ligand, Culture evaluation, Differentiation markers, Embryoid bodies, Expression profiling, Human embryonic germ cells, Primordial germ cells, STO feeder layer",

author = "Shamblott, {Michael J.} and Kerr, {Candace L.} and Joyce Axelman and Littlefield, {John W.} and Clark, {Gregory O.} and Patterson, {Ethan S.} and Addis, {Russell C.} and Kraszewski, {Jennifer N.} and Kent, {Kathleen C.} and Gearhart, {John D.}",

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AU - Shamblott, Michael J.

AU - Kerr, Candace L.

AU - Axelman, Joyce

AU - Littlefield, John W.

AU - Clark, Gregory O.

AU - Patterson, Ethan S.

AU - Addis, Russell C.

AU - Kraszewski, Jennifer N.

AU - Kent, Kathleen C.

AU - Gearhart, John D.

PY - 2014

Y1 - 2014

N2 - Human embryonic germ (EG) cells can be derived from primordial germ cells (PGCs) by using methods similar to those used to derive mouse EG cultures. Like mouse embryonic stem (ES) and EG cells, human EG cells require leukemia inhibitory factor (LIF) for proliferation as undifferentiated stem cells. Unlike mouse EG cells, however, human EG cells retain dependence on exogenous cytokines and factors supplied by the feeder layer, and spontaneously differentiate into embryoid bodies (EBs) more frequently. Although EBs are not pluripotent, they express phenotypic markers found on progenitor, precursor, and mature cells. Cells retaining a capacity for cell proliferation and express makers of multiple lineages can be isolated from EBs, and can be used in a variety of in vitro and in vivo differentiation paradigms. Current challenges are to match individual EB-derived (EBD) cultures to desired endpoints, and to enrich or purify specific populations of cells within EBD cultures.

AB - Human embryonic germ (EG) cells can be derived from primordial germ cells (PGCs) by using methods similar to those used to derive mouse EG cultures. Like mouse embryonic stem (ES) and EG cells, human EG cells require leukemia inhibitory factor (LIF) for proliferation as undifferentiated stem cells. Unlike mouse EG cells, however, human EG cells retain dependence on exogenous cytokines and factors supplied by the feeder layer, and spontaneously differentiate into embryoid bodies (EBs) more frequently. Although EBs are not pluripotent, they express phenotypic markers found on progenitor, precursor, and mature cells. Cells retaining a capacity for cell proliferation and express makers of multiple lineages can be isolated from EBs, and can be used in a variety of in vitro and in vivo differentiation paradigms. Current challenges are to match individual EB-derived (EBD) cultures to desired endpoints, and to enrich or purify specific populations of cells within EBD cultures.

KW - C-kit ligand

KW - Culture evaluation

KW - Differentiation markers

KW - Embryoid bodies

KW - Expression profiling

KW - Human embryonic germ cells

KW - Primordial germ cells

KW - STO feeder layer

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ER -

Derivation and Differentiation of Human Embryonic Germ Cells

Abstract

Keywords

ASJC Scopus subject areas

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