TY - JOUR
T1 - Dendritic Ca2+ channels characterized by recordings from isolated hippocampal dendritic segments
AU - Kavalali, Ege T.
AU - Zhuo, Min
AU - Bito, Haruhiko
AU - Tsien, Richard W.
N1 - Funding Information:
We thank Dr. Laszlo Nadasdi (Neurex Corp.) for ω-CTx-MVIIC and Dr. Yasuo Ihara (University of Tokyo) for anti-tau polyclonal antibodies. We are grateful to Karl Deisseroth and Jürgen Klingauf for critically reading the manuscript and all members of the Tsien laboratory for helpful discussions. This research was supported by the NIMH–Silvio Conte Center Grant MH48108 to R. W. T., an American Heart Association (California Affiliate) postdoctoral fellowship to E. T. K, and a Human Frontiers Science Program long-term fellowship to H. B.
PY - 1997/4
Y1 - 1997/4
N2 - Dendritic arbors are critical for the information processing capability of central neurons, but quantitative analysis of their membrane properties has been hampered by their geometrical complexity. Here, we have focused on an important source of Ca2+ entry in dendrites, the voltage-gated Ca2+ channels, by applying the whole-cell voltage-clamp technique to isolated dendritic segments ('dendrosomes') from rat hippocampal neurons. We found that low voltage-activated T-type Ca2+ channels provide a significantly larger fraction of the Ca2+ influx in dendrites than their counterparts in cell bodies. Surprisingly, 60%-70% of the high voltage-activated Ca2+ current in dendrosomes was N and P/Q type, and these channels were susceptible to neurotransmittar inhibition, suggesting a novel physiological role for G protein-regulated Ca2+ channel modulation in controlling dendritic excitability and Ca2+ signaling.
AB - Dendritic arbors are critical for the information processing capability of central neurons, but quantitative analysis of their membrane properties has been hampered by their geometrical complexity. Here, we have focused on an important source of Ca2+ entry in dendrites, the voltage-gated Ca2+ channels, by applying the whole-cell voltage-clamp technique to isolated dendritic segments ('dendrosomes') from rat hippocampal neurons. We found that low voltage-activated T-type Ca2+ channels provide a significantly larger fraction of the Ca2+ influx in dendrites than their counterparts in cell bodies. Surprisingly, 60%-70% of the high voltage-activated Ca2+ current in dendrosomes was N and P/Q type, and these channels were susceptible to neurotransmittar inhibition, suggesting a novel physiological role for G protein-regulated Ca2+ channel modulation in controlling dendritic excitability and Ca2+ signaling.
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U2 - 10.1016/S0896-6273(00)80305-0
DO - 10.1016/S0896-6273(00)80305-0
M3 - Article
C2 - 9136773
AN - SCOPUS:0030611546
SN - 0896-6273
VL - 18
SP - 651
EP - 663
JO - Neuron
JF - Neuron
IS - 4
ER -