Degradation of IF1 controls energy metabolism during osteogenic differentiation of stem cells

María Sánchez-Aragó; Javier García-Bermúdez; Inmaculada Martínez-Reyes; Fulvio Santacatterina; José M. Cuezva

doi:10.1038/embor.2013.72

Degradation of IF1 controls energy metabolism during osteogenic differentiation of stem cells

María Sánchez-Aragó, Javier García-Bermúdez, Inmaculada Martínez-Reyes, Fulvio Santacatterina, José M. Cuezva

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Differentiation of human mesenchymal stem cells (hMSCs) requires the rewiring of energy metabolism. Herein, we demonstrate that the ATPase inhibitory factor 1 (IF1) is expressed in hMSCs and in prostate and colon stem cells but is not expressed in the differentiated cells. IF1 inhibits oxidative phosphorylation and regulates the activity of aerobic glycolysis in hMSCs. Silencing of IF1 in hMSCs mimics the metabolic changes observed in osteocytes and accelerates cellular differentiation. Activation of IF1 degradation acts as the switch that regulates energy metabolism during differentiation. We conclude that IF1 is a stemness marker important for maintaining the quiescence state.

Original language	English (US)
Pages (from-to)	638-644
Number of pages	7
Journal	EMBO Reports
Volume	14
Issue number	7
DOIs	https://doi.org/10.1038/embor.2013.72
State	Published - Jun 2013
Externally published	Yes

Keywords

ATPase inhibitory factor 1
H-ATP synthase
cellular differentiation
mitochondria
protein degradation

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

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10.1038/embor.2013.72

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abstract = "Differentiation of human mesenchymal stem cells (hMSCs) requires the rewiring of energy metabolism. Herein, we demonstrate that the ATPase inhibitory factor 1 (IF1) is expressed in hMSCs and in prostate and colon stem cells but is not expressed in the differentiated cells. IF1 inhibits oxidative phosphorylation and regulates the activity of aerobic glycolysis in hMSCs. Silencing of IF1 in hMSCs mimics the metabolic changes observed in osteocytes and accelerates cellular differentiation. Activation of IF1 degradation acts as the switch that regulates energy metabolism during differentiation. We conclude that IF1 is a stemness marker important for maintaining the quiescence state.",

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AU - Sánchez-Aragó, María

AU - García-Bermúdez, Javier

AU - Martínez-Reyes, Inmaculada

AU - Santacatterina, Fulvio

AU - Cuezva, José M.

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AB - Differentiation of human mesenchymal stem cells (hMSCs) requires the rewiring of energy metabolism. Herein, we demonstrate that the ATPase inhibitory factor 1 (IF1) is expressed in hMSCs and in prostate and colon stem cells but is not expressed in the differentiated cells. IF1 inhibits oxidative phosphorylation and regulates the activity of aerobic glycolysis in hMSCs. Silencing of IF1 in hMSCs mimics the metabolic changes observed in osteocytes and accelerates cellular differentiation. Activation of IF1 degradation acts as the switch that regulates energy metabolism during differentiation. We conclude that IF1 is a stemness marker important for maintaining the quiescence state.

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Degradation of IF1 controls energy metabolism during osteogenic differentiation of stem cells

Abstract

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