Deformable registration of histological cancer margins to gross hyperspectral images using demons

Martin Halicek, James V. Little, Xu Wang, Zhuo Georgia Chen, Mihir Patel, Christopher C. Griffith, Mark W. El-Deiry, Nabil F. Saba, Amy Y. Chen, Baowei Fei

Research output: Chapter in Book/Report/Conference proceedingConference contribution

7 Scopus citations


Hyperspectral imaging (HSI), a non-contact optical imaging technique, has been recently used along with machine learning technique to provide diagnostic information about ex-vivo surgical specimens for optical biopsy. The computer-Aided diagnostic approach requires accurate ground truths for both training and validation. This study details a processing pipeline for registering the cancer-normal margin from a digitized histological image to the gross-level HSI of a tissue specimen. Our work incorporates an initial affine and control-point registration followed by a deformable Demons-based registration of the moving mask obtained from the histological image to the fixed mask made from the HS image. To assess registration quality, Dice similarity coefficient (DSC) measures the image overlap, visual inspection is used to evaluate the margin, and average target registration error (TRE) of needle-bored holes measures the registration error between the histologic and HSI images. Excised tissue samples from seventeen patients, 11 head and neck squamous cell carcinoma (HNSCCa) and 6 thyroid carcinoma, were registered according to the proposed method. Three registered specimens are illustrated in this paper, which demonstrate the efficacy of the registration workflow. Further work is required to apply the technique to more patient data and investigate the ability of this procedure to produce suitable gold standards for machine learning validation.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2018
Subtitle of host publicationDigital Pathology
EditorsMetin N. Gurcan, John E. Tomaszewski
ISBN (Electronic)9781510616516
StatePublished - 2018
Externally publishedYes
EventMedical Imaging 2018: Digital Pathology - Houston, United States
Duration: Feb 11 2018Feb 12 2018

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceMedical Imaging 2018: Digital Pathology
Country/TerritoryUnited States


  • Demons registration
  • Digital histology
  • Head and neck cancer surgery
  • Hyperspectral imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging


Dive into the research topics of 'Deformable registration of histological cancer margins to gross hyperspectral images using demons'. Together they form a unique fingerprint.

Cite this