TY - JOUR
T1 - Deciphering and disrupting PIEZO1-TMEM16F interplay in hereditary xerocytosis
AU - Liang, Pengfei
AU - Zhang, Yang
AU - Wan, Yui Chun S.
AU - Ma, Shang
AU - Dong, Ping
AU - Lowry, Augustus J.
AU - Francis, Samuel J.
AU - Khandelwal, Sanjay
AU - Delahunty, Martha
AU - Telen, Marilyn J.
AU - Strouse, John J.
AU - Arepally, Gowthami M.
AU - Yang, Huanghe
N1 - Publisher Copyright:
© 2024 American Society of Hematology
PY - 2024/1/25
Y1 - 2024/1/25
N2 - Cell-surface exposure of phosphatidylserine (PS) is essential for phagocytic clearance and blood clotting. Although a calcium-activated phospholipid scramblase (CaPLSase) has long been proposed to mediate PS exposure in red blood cells (RBCs), its identity, activation mechanism, and role in RBC biology and disease remain elusive. Here, we demonstrate that TMEM16F, the long-sought-after RBC CaPLSase, is activated by calcium influx through the mechanosensitive channel PIEZO1 in RBCs. PIEZO1-TMEM16F functional coupling is enhanced in RBCs from individuals with hereditary xerocytosis (HX), an RBC disorder caused by PIEZO1 gain-of-function channelopathy. Enhanced PIEZO1-TMEM16F coupling leads to an increased propensity to expose PS, which may serve as a key risk factor for HX clinical manifestations including anemia, splenomegaly, and postsplenectomy thrombosis. Spider toxin GsMTx-4 and antigout medication benzbromarone inhibit PIEZO1, preventing force-induced echinocytosis, hemolysis, and PS exposure in HX RBCs. Our study thus reveals an activation mechanism of TMEM16F CaPLSase and its pathophysiological function in HX, providing insights into potential treatment.
AB - Cell-surface exposure of phosphatidylserine (PS) is essential for phagocytic clearance and blood clotting. Although a calcium-activated phospholipid scramblase (CaPLSase) has long been proposed to mediate PS exposure in red blood cells (RBCs), its identity, activation mechanism, and role in RBC biology and disease remain elusive. Here, we demonstrate that TMEM16F, the long-sought-after RBC CaPLSase, is activated by calcium influx through the mechanosensitive channel PIEZO1 in RBCs. PIEZO1-TMEM16F functional coupling is enhanced in RBCs from individuals with hereditary xerocytosis (HX), an RBC disorder caused by PIEZO1 gain-of-function channelopathy. Enhanced PIEZO1-TMEM16F coupling leads to an increased propensity to expose PS, which may serve as a key risk factor for HX clinical manifestations including anemia, splenomegaly, and postsplenectomy thrombosis. Spider toxin GsMTx-4 and antigout medication benzbromarone inhibit PIEZO1, preventing force-induced echinocytosis, hemolysis, and PS exposure in HX RBCs. Our study thus reveals an activation mechanism of TMEM16F CaPLSase and its pathophysiological function in HX, providing insights into potential treatment.
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U2 - 10.1182/blood.2023021465
DO - 10.1182/blood.2023021465
M3 - Article
C2 - 38033286
AN - SCOPUS:85180568747
SN - 0006-4971
VL - 143
SP - 357
EP - 369
JO - Blood
JF - Blood
IS - 4
ER -