Cytochrome P450-dependent arachidonate metabolites affect renal transport in the rabbit

B. Escalante; D. Erlij; J. R. Falck; J. C. McGiff

doi:10.1097/00005344-199322002-00034

Cytochrome P450-dependent arachidonate metabolites affect renal transport in the rabbit

B. Escalante, D. Erlij, J. R. Falck, J. C. McGiff

Biochemistry

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Arachidonic acid (AA) inhibited ⁸⁶Rb uptake in a dose-dependent manner in rabbit medullary tubules representing the thick ascending limb of the loop of Henle (mTALH). We have shown that CoCl₂ treatment of rabbits reduced renal AA metabolism by cytochrome P450-dependent enzymes (P450). After CoCl₂ treatment, AA was without effect on ⁸⁶Rb uptake in mTALH cells. However, the two principal P450-AA metabolites produced by the mTALH, 20-HETE and 20- COOH-AA, inhibited ⁸⁶Rb uptake in a dose-dependent manner in mTALH cells whether obtained from control or CoCl₂-treated rabbits. These data support our hypothesis that CoCl₂ treatment impairs ion transport function of mTALH cells by blocking cytochrome P450-AA metabolism, a blockade circumvented by the relevant P450-AA metabolites.

Original language	English (US)
Pages (from-to)	S106-S108
Journal	Journal of Cardiovascular Pharmacology
Volume	22
Issue number	SUPPL. 2
DOIs	https://doi.org/10.1097/00005344-199322002-00034
State	Published - 1993

Keywords

20- Hydroxyeicosatetraenoic acid
Arachidonic acid
Cytochrome P450
Medullary thick ascending limb of the loop of Henle
Rubidium uptake

ASJC Scopus subject areas

Pharmacology
Cardiology and Cardiovascular Medicine

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10.1097/00005344-199322002-00034

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title = "Cytochrome P450-dependent arachidonate metabolites affect renal transport in the rabbit",

abstract = "Arachidonic acid (AA) inhibited 86Rb uptake in a dose-dependent manner in rabbit medullary tubules representing the thick ascending limb of the loop of Henle (mTALH). We have shown that CoCl2 treatment of rabbits reduced renal AA metabolism by cytochrome P450-dependent enzymes (P450). After CoCl2 treatment, AA was without effect on 86Rb uptake in mTALH cells. However, the two principal P450-AA metabolites produced by the mTALH, 20-HETE and 20- COOH-AA, inhibited 86Rb uptake in a dose-dependent manner in mTALH cells whether obtained from control or CoCl2-treated rabbits. These data support our hypothesis that CoCl2 treatment impairs ion transport function of mTALH cells by blocking cytochrome P450-AA metabolism, a blockade circumvented by the relevant P450-AA metabolites.",

keywords = "20- Hydroxyeicosatetraenoic acid, Arachidonic acid, Cytochrome P450, Medullary thick ascending limb of the loop of Henle, Rubidium uptake",

author = "B. Escalante and D. Erlij and Falck, {J. R.} and McGiff, {J. C.}",

year = "1993",

doi = "10.1097/00005344-199322002-00034",

language = "English (US)",

volume = "22",

pages = "S106--S108",

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T1 - Cytochrome P450-dependent arachidonate metabolites affect renal transport in the rabbit

AU - Escalante, B.

AU - Erlij, D.

AU - Falck, J. R.

AU - McGiff, J. C.

PY - 1993

Y1 - 1993

N2 - Arachidonic acid (AA) inhibited 86Rb uptake in a dose-dependent manner in rabbit medullary tubules representing the thick ascending limb of the loop of Henle (mTALH). We have shown that CoCl2 treatment of rabbits reduced renal AA metabolism by cytochrome P450-dependent enzymes (P450). After CoCl2 treatment, AA was without effect on 86Rb uptake in mTALH cells. However, the two principal P450-AA metabolites produced by the mTALH, 20-HETE and 20- COOH-AA, inhibited 86Rb uptake in a dose-dependent manner in mTALH cells whether obtained from control or CoCl2-treated rabbits. These data support our hypothesis that CoCl2 treatment impairs ion transport function of mTALH cells by blocking cytochrome P450-AA metabolism, a blockade circumvented by the relevant P450-AA metabolites.

AB - Arachidonic acid (AA) inhibited 86Rb uptake in a dose-dependent manner in rabbit medullary tubules representing the thick ascending limb of the loop of Henle (mTALH). We have shown that CoCl2 treatment of rabbits reduced renal AA metabolism by cytochrome P450-dependent enzymes (P450). After CoCl2 treatment, AA was without effect on 86Rb uptake in mTALH cells. However, the two principal P450-AA metabolites produced by the mTALH, 20-HETE and 20- COOH-AA, inhibited 86Rb uptake in a dose-dependent manner in mTALH cells whether obtained from control or CoCl2-treated rabbits. These data support our hypothesis that CoCl2 treatment impairs ion transport function of mTALH cells by blocking cytochrome P450-AA metabolism, a blockade circumvented by the relevant P450-AA metabolites.

KW - 20- Hydroxyeicosatetraenoic acid

KW - Arachidonic acid

KW - Cytochrome P450

KW - Medullary thick ascending limb of the loop of Henle

KW - Rubidium uptake

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VL - 22

SP - S106-S108

JO - Journal of Cardiovascular Pharmacology

JF - Journal of Cardiovascular Pharmacology

IS - SUPPL. 2

ER -

Cytochrome P450-dependent arachidonate metabolites affect renal transport in the rabbit

Abstract

Keywords

ASJC Scopus subject areas

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