This chapter discusses the cytochrome P450 arachidonic acid epoxygenase. The epoxygenase regio- and stereoselectivity of oxygenation is under the control of the cytochrome P450 protein catalyst. The potential functional significance of this metabolic pathway has been highlighted by (a) the potent biological activities of the epoxyeicosatrienoic acids (EETs), and (b) the demonstration of a role for the hemoprotein in the in vivo metabolism of endogenous arachidonic acid. The enantioselective nature of the endogenous EET pools present in rat liver and human kidney established the epoxygenase as an additional member of the “arachidonate metabolic cascade.” This chapter discusses the methodology developed in laboratories for the regio- and stereochemical characterization of the cytochrome P450 epoxygenase metabolites generated in vitro by enzymatic incubations or present in vivo in extracts isolated from whole organs.
ASJC Scopus subject areas
- Molecular Biology