Cyclic changes in metabolic state during the life of a yeast cell

Benjamin P. Tu, Rachel E. Mohler, Jessica C. Liu, Kenneth M. Dombek, Elton T. Young, Robert E. Synovec, Steven L. McKnight

Research output: Contribution to journalArticlepeer-review

204 Scopus citations


Budding yeast undergo robust oscillations in oxygen consumption during continuous growth in a nutrient-limited environment. Using liquid chromatography-mass spectrometry and comprehensive 2D gas chromatography-mass spectrometry-based metabolite profiling methods, we have determined that the intracellular concentrations of many metabolites change periodically as a function of these metabolic cycles. These results reveal the logic of cellular metabolism during different phases of the life of a yeast cell. They may further indicate that oscillation in the abundance of key metabolites might help control the temporal regulation of cellular processes and the establishment of a cycle. Such oscillations in metabolic state might occur during the course of other biological cycles.

Original languageEnglish (US)
Pages (from-to)16886-16891
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number43
StatePublished - Oct 23 2007


  • Gas chromatography-mass spectrometry
  • Liquid chromatography-mass spectrometry
  • Metabolic cycle
  • Metabolite profiling

ASJC Scopus subject areas

  • General


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