TY - JOUR
T1 - CUG repeats present in myotonin kinase RNA form metastable 'slippery' hairpins
AU - Napierała, Marek
AU - Krzyzosiak, Włodzimierz J.
PY - 1997/12/5
Y1 - 1997/12/5
N2 - We show that CUG repeats form 'slippery' hairpins in their natural sequence context of the myotonin kinase gene transcript. This novel type of RNA structure is characterized by strong S1 and T1 nuclease and lead cleavages in the terminal loop and by mild lead cleavages in the hairpin stem. The latter effect indicates a relaxed metastable structure of the stem. (CUG)5 repeats do not form any detectable secondary structure, whereas hairpins of increasing stability are formed by (CUG)11, (CUG)21, and (CUG)49. The potential role of the RNA hairpin structure in the pathogenesis of myotonic dystrophy is discussed.
AB - We show that CUG repeats form 'slippery' hairpins in their natural sequence context of the myotonin kinase gene transcript. This novel type of RNA structure is characterized by strong S1 and T1 nuclease and lead cleavages in the terminal loop and by mild lead cleavages in the hairpin stem. The latter effect indicates a relaxed metastable structure of the stem. (CUG)5 repeats do not form any detectable secondary structure, whereas hairpins of increasing stability are formed by (CUG)11, (CUG)21, and (CUG)49. The potential role of the RNA hairpin structure in the pathogenesis of myotonic dystrophy is discussed.
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U2 - 10.1074/jbc.272.49.31079
DO - 10.1074/jbc.272.49.31079
M3 - Article
C2 - 9388259
AN - SCOPUS:1842406027
SN - 0021-9258
VL - 272
SP - 31079
EP - 31085
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 49
ER -