Covalently bound fatty acids in membrane proteins of some sponges

Eser Ayanoglu; Michele Rizzolio; Stacey Beaulieu; Julie Roberts; Orhan Oz; Carl Djerassi

doi:10.1016/0305-0491(90)90063-Y

Covalently bound fatty acids in membrane proteins of some sponges

Eser Ayanoglu, Michele Rizzolio, Stacey Beaulieu, Julie Roberts, Orhan Oz, Carl Djerassi

Research output: Contribution to journal › Article › peer-review

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Abstract

1. 1. Fatty acids covalently bound to membrane proteins were studied in the marine sponges Spheciospongia confoederata, Microciona prolifera and Tethya aurantia. 2. 2. Cell membrane isolates obtained by successive centrifugation and ultracentrifugation steps were subjected to exhaustive lipid extraction by organic solvents. 3. 3. After delipidation, fatty acids covalently bound to membrane proteins were obtained by hydrolysis. 4. 4. While total lipids from sponge extracts as well as from the organic extracts of the membrane delipidization procedure contained very long chain (C_24-30) fatty acids as their major constituents, fatty acids covalently bound to proteins were predominantly of the conventional (C_16-18) type.

Original language	English (US)
Pages (from-to)	597-603
Number of pages	7
Journal	Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume	96
Issue number	3
DOIs	https://doi.org/10.1016/0305-0491(90)90063-Y
State	Published - 1990

ASJC Scopus subject areas

Biochemistry
Physiology
Molecular Biology

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@article{96c892a2e4774c7eb312cbefd1fcb6f6,

title = "Covalently bound fatty acids in membrane proteins of some sponges",

abstract = "1. 1. Fatty acids covalently bound to membrane proteins were studied in the marine sponges Spheciospongia confoederata, Microciona prolifera and Tethya aurantia. 2. 2. Cell membrane isolates obtained by successive centrifugation and ultracentrifugation steps were subjected to exhaustive lipid extraction by organic solvents. 3. 3. After delipidation, fatty acids covalently bound to membrane proteins were obtained by hydrolysis. 4. 4. While total lipids from sponge extracts as well as from the organic extracts of the membrane delipidization procedure contained very long chain (C24-30) fatty acids as their major constituents, fatty acids covalently bound to proteins were predominantly of the conventional (C16-18) type.",

author = "Eser Ayanoglu and Michele Rizzolio and Stacey Beaulieu and Julie Roberts and Orhan Oz and Carl Djerassi",

note = "Funding Information: Acknowledgements--We thank our laboratory colleagues Christopher Silva, Dr Russell Kerr and Max Hoberg for the collection of sponge samples, Frances Thomas for electron microscopy, and Professor Wray Huestis as well as Dr Victoria Levy for useful discussions. Financial support from NSF (DMB-8606249) is gratefully acknowledged.",

year = "1990",

doi = "10.1016/0305-0491(90)90063-Y",

language = "English (US)",

volume = "96",

pages = "597--603",

journal = "Comparative Biochemistry and Physiology -- Part B: Biochemistry and",

issn = "0305-0491",

publisher = "Elsevier Inc.",

number = "3",

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TY - JOUR

T1 - Covalently bound fatty acids in membrane proteins of some sponges

AU - Ayanoglu, Eser

AU - Rizzolio, Michele

AU - Beaulieu, Stacey

AU - Roberts, Julie

AU - Oz, Orhan

AU - Djerassi, Carl

N1 - Funding Information: Acknowledgements--We thank our laboratory colleagues Christopher Silva, Dr Russell Kerr and Max Hoberg for the collection of sponge samples, Frances Thomas for electron microscopy, and Professor Wray Huestis as well as Dr Victoria Levy for useful discussions. Financial support from NSF (DMB-8606249) is gratefully acknowledged.

PY - 1990

Y1 - 1990

N2 - 1. 1. Fatty acids covalently bound to membrane proteins were studied in the marine sponges Spheciospongia confoederata, Microciona prolifera and Tethya aurantia. 2. 2. Cell membrane isolates obtained by successive centrifugation and ultracentrifugation steps were subjected to exhaustive lipid extraction by organic solvents. 3. 3. After delipidation, fatty acids covalently bound to membrane proteins were obtained by hydrolysis. 4. 4. While total lipids from sponge extracts as well as from the organic extracts of the membrane delipidization procedure contained very long chain (C24-30) fatty acids as their major constituents, fatty acids covalently bound to proteins were predominantly of the conventional (C16-18) type.

AB - 1. 1. Fatty acids covalently bound to membrane proteins were studied in the marine sponges Spheciospongia confoederata, Microciona prolifera and Tethya aurantia. 2. 2. Cell membrane isolates obtained by successive centrifugation and ultracentrifugation steps were subjected to exhaustive lipid extraction by organic solvents. 3. 3. After delipidation, fatty acids covalently bound to membrane proteins were obtained by hydrolysis. 4. 4. While total lipids from sponge extracts as well as from the organic extracts of the membrane delipidization procedure contained very long chain (C24-30) fatty acids as their major constituents, fatty acids covalently bound to proteins were predominantly of the conventional (C16-18) type.

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U2 - 10.1016/0305-0491(90)90063-Y

DO - 10.1016/0305-0491(90)90063-Y

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SN - 0305-0491

VL - 96

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EP - 603

JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and

JF - Comparative Biochemistry and Physiology -- Part B: Biochemistry and

IS - 3

ER -

Covalently bound fatty acids in membrane proteins of some sponges

Abstract

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