TY - JOUR
T1 - Covalently bound fatty acids in membrane proteins of some sponges
AU - Ayanoglu, Eser
AU - Rizzolio, Michele
AU - Beaulieu, Stacey
AU - Roberts, Julie
AU - Oz, Orhan
AU - Djerassi, Carl
N1 - Funding Information:
Acknowledgements--We thank our laboratory colleagues Christopher Silva, Dr Russell Kerr and Max Hoberg for the collection of sponge samples, Frances Thomas for electron microscopy, and Professor Wray Huestis as well as Dr Victoria Levy for useful discussions. Financial support from NSF (DMB-8606249) is gratefully acknowledged.
PY - 1990
Y1 - 1990
N2 - 1. 1. Fatty acids covalently bound to membrane proteins were studied in the marine sponges Spheciospongia confoederata, Microciona prolifera and Tethya aurantia. 2. 2. Cell membrane isolates obtained by successive centrifugation and ultracentrifugation steps were subjected to exhaustive lipid extraction by organic solvents. 3. 3. After delipidation, fatty acids covalently bound to membrane proteins were obtained by hydrolysis. 4. 4. While total lipids from sponge extracts as well as from the organic extracts of the membrane delipidization procedure contained very long chain (C24-30) fatty acids as their major constituents, fatty acids covalently bound to proteins were predominantly of the conventional (C16-18) type.
AB - 1. 1. Fatty acids covalently bound to membrane proteins were studied in the marine sponges Spheciospongia confoederata, Microciona prolifera and Tethya aurantia. 2. 2. Cell membrane isolates obtained by successive centrifugation and ultracentrifugation steps were subjected to exhaustive lipid extraction by organic solvents. 3. 3. After delipidation, fatty acids covalently bound to membrane proteins were obtained by hydrolysis. 4. 4. While total lipids from sponge extracts as well as from the organic extracts of the membrane delipidization procedure contained very long chain (C24-30) fatty acids as their major constituents, fatty acids covalently bound to proteins were predominantly of the conventional (C16-18) type.
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U2 - 10.1016/0305-0491(90)90063-Y
DO - 10.1016/0305-0491(90)90063-Y
M3 - Article
AN - SCOPUS:0025279351
SN - 1096-4959
VL - 96
SP - 597
EP - 603
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
IS - 3
ER -