TY - JOUR
T1 - Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme
AU - Nishimura, Noriyuki
AU - Tsuchiya, Wataru
AU - Moresco, James J.
AU - Hayashi, Yuki
AU - Satoh, Kouji
AU - Kaiwa, Nahomi
AU - Irisa, Tomoko
AU - Kinoshita, Toshinori
AU - Schroeder, Julian I.
AU - Yates, John R.
AU - Hirayama, Takashi
AU - Yamazaki, Toshimasa
N1 - Funding Information:
We are grateful to Dr. Claire Delahunty and to Mr. Nathaniel Duncan for critical reading and comments on the manuscript. We thank Drs. Wim Soppe and Kazumi Nakabayashi for dog1-2 seeds, Dr. Paul Verslues for hai1-2, aip1-1 and hai3-1 seeds, Dr. Manabu Nakayama for pACTGW-attR and pASGW-attR vectors, Dr. Taku Demura for pH35YG vector, Dr. Yasuo Niwa for sGFP vector, ABRC for providing plant materials or reagents, Dr. Mitsuhiro Miyazawa for measuring CD spectra, and Ms. Izumi Odano for technical assistance. This research was supported by MEXT/JSPS KAKENHI (Grant Numbers 23688044, 26712030, and 23119521) and MEXT as part of Joint Research Program implemented at the Institute of Plant Science and Resources, Okayama University in Japan (2608, 2707, and 2805) to N.N., MEXT KAKENHI (15H05956) to T.K., National Institutes of Health (GM060396) to J.I.S., the National Institute of General Medical Sciences (8 P41 GM103533) to J.J.M and J.R.Y., and the National Institute of Agrobiological Sciences Strategic Fund to T.Y.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes, but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system.
AB - Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes, but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system.
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U2 - 10.1038/s41467-018-04437-9
DO - 10.1038/s41467-018-04437-9
M3 - Article
C2 - 29875377
AN - SCOPUS:85048307618
SN - 2041-1723
VL - 9
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 2132
ER -