TY - JOUR
T1 - Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor
AU - Gazdar, A. F.
AU - Chick, W. L.
AU - Oie, H. K.
AU - Sims, H. L.
AU - King, D. L.
AU - Weir, G. C.
AU - Lauris, V.
PY - 1980
Y1 - 1980
N2 - Continuous cell lines that secrete both insulin and somatostatin were established by two cooperating groups of investigators from a serially transplantable, radiation-induced, rat islet cell tumor. The cell lines, named RIN-r and RIN-m, were initiated from tumors maintained in inbred rats or in athymic nude mice, respectively. The cultured cells are epithelioid, free of fibroblast contamination, and can be cloned. They have a hypodiploid chromosome number, are tumorigenic, and possess amine-handling properties, including high levels of L-dopa decarboxylase and formaldehyde-induced fluorescence. Preliminary analysis of clones revealed a spectrum of insulin secretion from undetectable to relatively high. These clonal cell lines provide important systems to study the biology of insulin and somatostatin.
AB - Continuous cell lines that secrete both insulin and somatostatin were established by two cooperating groups of investigators from a serially transplantable, radiation-induced, rat islet cell tumor. The cell lines, named RIN-r and RIN-m, were initiated from tumors maintained in inbred rats or in athymic nude mice, respectively. The cultured cells are epithelioid, free of fibroblast contamination, and can be cloned. They have a hypodiploid chromosome number, are tumorigenic, and possess amine-handling properties, including high levels of L-dopa decarboxylase and formaldehyde-induced fluorescence. Preliminary analysis of clones revealed a spectrum of insulin secretion from undetectable to relatively high. These clonal cell lines provide important systems to study the biology of insulin and somatostatin.
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U2 - 10.1073/pnas.77.6.3519
DO - 10.1073/pnas.77.6.3519
M3 - Article
C2 - 6106192
AN - SCOPUS:0009611219
SN - 0027-8424
VL - 77
SP - 3519
EP - 3523
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 6 I
ER -