TY - JOUR
T1 - Contact Repulsion Controls the Dispersion and Final Distribution of Cajal-Retzius Cells
AU - Villar-Cerviño, Verona
AU - Molano-Mazón, Manuel
AU - Catchpole, Timothy
AU - Valdeolmillos, Miguel
AU - Henkemeyer, Mark
AU - Martínez, Luis M.
AU - Borrell, Víctor
AU - Marín, Oscar
N1 - Funding Information:
We thank A. Casillas for excellent technical assistance, T. Gil and M. Pérez for lab support, U. Drescher, E. Herrera, J.L R. Rubenstein, A. Wanaka, and D. Wilkinson for plasmids, and G. Fishell (RCE) and J.Z. Huang (Calretinin-Cre) for mouse colonies. We are grateful to B. Hassan, M. Maravall, M.A. Nieto for critical reading of the manuscript and members of the Borrell, Marín, and Rico laboratories for stimulating discussions and ideas. This work was supported by grants from Spanish Ministry of Science and Innovation SAF2011-28845 and CONSOLIDER CSD2007-00023 to O.M. and from the National Institutes of Health (R01 MH66332) to M.H. V.V.-C. was a “Junta de Ampliación de Estudios” (JAE) postdoctoral fellow from the Consejo Superior de Investigaciones Científicas and is now a “Juan de la Cierva” postdoctoral fellow from the Ministerio de Economía e Innovación.
PY - 2013/2/6
Y1 - 2013/2/6
N2 - Cajal-Retzius (CR) cells play a fundamental role in the development of the mammalian cerebral cortex. They control the formation of cortical layers by regulating the migration of pyramidal cells through the release of Reelin. The function of CR cells critically depends on their regular distribution throughout the surface of the cortex, but little is known about the events controlling this phenomenon. Using time-lapse video microscopy in vivo and in vitro, we found that movement of CR cells is regulated by repulsive interactions, which leads to their random dispersion throughout the cortical surface. Mathematical modeling reveals that contact repulsion is both necessary and sufficient for this process, which demonstrates that complex neuronal assemblies may emerge during development through stochastic events. At the molecular level, we found that contact repulsion is mediated by Eph/ephrin interactions. Our observations reveal a mechanism that controls the even distribution of neurons in the developing brain
AB - Cajal-Retzius (CR) cells play a fundamental role in the development of the mammalian cerebral cortex. They control the formation of cortical layers by regulating the migration of pyramidal cells through the release of Reelin. The function of CR cells critically depends on their regular distribution throughout the surface of the cortex, but little is known about the events controlling this phenomenon. Using time-lapse video microscopy in vivo and in vitro, we found that movement of CR cells is regulated by repulsive interactions, which leads to their random dispersion throughout the cortical surface. Mathematical modeling reveals that contact repulsion is both necessary and sufficient for this process, which demonstrates that complex neuronal assemblies may emerge during development through stochastic events. At the molecular level, we found that contact repulsion is mediated by Eph/ephrin interactions. Our observations reveal a mechanism that controls the even distribution of neurons in the developing brain
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U2 - 10.1016/j.neuron.2012.11.023
DO - 10.1016/j.neuron.2012.11.023
M3 - Article
C2 - 23395373
AN - SCOPUS:84873312214
SN - 0896-6273
VL - 77
SP - 457
EP - 471
JO - Neuron
JF - Neuron
IS - 3
ER -