TY - JOUR
T1 - Conservation of small RNA pathways in platypus
AU - Murchison, Elizabeth P.
AU - Kheradpour, Pouya
AU - Sachidanandam, Ravi
AU - Smith, Carly
AU - Hodges, Emily
AU - Xuan, Zhenyu
AU - Kellis, Manolis
AU - Grützner, Frank
AU - Stark, Alexander
AU - Hannon, Gregory J.
PY - 2008/6
Y1 - 2008/6
N2 - Small RNA pathways play evolutionarily conserved roles in gene regulation and defense from parasitic nucleic acids. The character and expression patterns of small RNAs show conservation throughout animal lineages, but specific animal clades also show variations on these recurring themes, including species-specific small RNAs. The monotremes, with only platypus and four species of echidna as extant members, represent the basal branch of the mammalian lineage. Here, we examine the small RNA pathways of monotremes by deep sequencing of six platypus and echidna tissues. We find that highly conserved microRNA species display their signature tissue-specific expression patterns. In addition, we find a large rapidly evolving cluster of microRNAs on platypus chromosome X1, which is unique to monotremes. Platypus and echidna testes contain a robust Piwi-interacting (piRNA) system, which appears to be participating in ongoing transposon defense.
AB - Small RNA pathways play evolutionarily conserved roles in gene regulation and defense from parasitic nucleic acids. The character and expression patterns of small RNAs show conservation throughout animal lineages, but specific animal clades also show variations on these recurring themes, including species-specific small RNAs. The monotremes, with only platypus and four species of echidna as extant members, represent the basal branch of the mammalian lineage. Here, we examine the small RNA pathways of monotremes by deep sequencing of six platypus and echidna tissues. We find that highly conserved microRNA species display their signature tissue-specific expression patterns. In addition, we find a large rapidly evolving cluster of microRNAs on platypus chromosome X1, which is unique to monotremes. Platypus and echidna testes contain a robust Piwi-interacting (piRNA) system, which appears to be participating in ongoing transposon defense.
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U2 - 10.1101/gr.073056.107
DO - 10.1101/gr.073056.107
M3 - Article
C2 - 18463306
AN - SCOPUS:43749086317
SN - 1088-9051
VL - 18
SP - 995
EP - 1004
JO - Genome Research
JF - Genome Research
IS - 6
ER -