TY - JOUR
T1 - Consensus report on flow cytometry for platelet function testing in thrombocytopenic patients
T2 - communication from the SSC of the ISTH
AU - The FC-PFT in TP study group
AU - Jourdi, Georges
AU - Ramström, Sofia
AU - Sharma, Ruchika
AU - Bakchoul, Tamam
AU - Lordkipanidzé, Marie
N1 - Funding Information:
The authors are grateful for the administrative support provided by Cary Clark, Rebekah Perry, and Shannon Brooks from the ISTH headquarters. S.R. G.J. R.S. T.B. and M.L. conceived and designed the study. S.R. G.J. and R.S. carried out the study. G.J. drafted the manuscript. T.B. and M.L. oversaw the projects of the SSC on Platelet Immunology (T.B.) and Platelet Physiology (M.L.) as Chairs. All authors reviewed and approved the manuscript. M.L. has received speaker fees from Bayer and Diagnostica Stago; has participated in industry-funded trials from Idorsia; has served on advisory boards for Servier and JAMP/Orimed Pharma; and has received in-kind support for investigator-initiated grants from Fujimori Kogyo. Other authors declare no conflicts of interest.
Publisher Copyright:
© 2023
PY - 2023/10
Y1 - 2023/10
N2 - Background: Platelet count alone does not reliably predict bleeding risk, suggesting platelet function is important to monitor in patients with thrombocytopenia. There is still an unmet need for improved platelet function diagnostics in patients with low platelet count in many clinical situations. Flow cytometry is a promising tool allowing reliable platelet function study in this setting. Objectives: The goal of this joint project between the International Society on Thrombosis and Haemostasis (ISTH) Scientific Standardization Committee (SSC) Subcommittees on Platelet Physiology and Platelet Immunology is to provide expert consensus guidance on the use of flow cytometry for the evaluation of platelet function, particularly activation, in patients with low platelet counts. Methods: A literature review was performed to identify relevant questions and areas of interest. An electronic expression of interest form was thereafter announced on the ISTH webpage, followed by a survey encompassing 37 issues regarding preanalytical, analytical, postanalytical, and performance aspects. Areas of disagreement or uncertainty were identified and formed the basis for 2 focus group discussions. Results: Consensus recommendations relative to patient sample collection, preanalytical variables, sample type, platelet-count cutoff, any potential specific modification of the standard flow cytometry protocol, and results expression and reporting are proposed based on the current practices of experts in the field as well as on literature review. Conclusion: The proposed consensus recommendations would allow standardization of protocols in upcoming clinical studies. The clinical utility of platelet function testing using flow cytometry to predict bleeding risk still needs rigorous multicenter outcome studies in patients with thrombocytopenia.
AB - Background: Platelet count alone does not reliably predict bleeding risk, suggesting platelet function is important to monitor in patients with thrombocytopenia. There is still an unmet need for improved platelet function diagnostics in patients with low platelet count in many clinical situations. Flow cytometry is a promising tool allowing reliable platelet function study in this setting. Objectives: The goal of this joint project between the International Society on Thrombosis and Haemostasis (ISTH) Scientific Standardization Committee (SSC) Subcommittees on Platelet Physiology and Platelet Immunology is to provide expert consensus guidance on the use of flow cytometry for the evaluation of platelet function, particularly activation, in patients with low platelet counts. Methods: A literature review was performed to identify relevant questions and areas of interest. An electronic expression of interest form was thereafter announced on the ISTH webpage, followed by a survey encompassing 37 issues regarding preanalytical, analytical, postanalytical, and performance aspects. Areas of disagreement or uncertainty were identified and formed the basis for 2 focus group discussions. Results: Consensus recommendations relative to patient sample collection, preanalytical variables, sample type, platelet-count cutoff, any potential specific modification of the standard flow cytometry protocol, and results expression and reporting are proposed based on the current practices of experts in the field as well as on literature review. Conclusion: The proposed consensus recommendations would allow standardization of protocols in upcoming clinical studies. The clinical utility of platelet function testing using flow cytometry to predict bleeding risk still needs rigorous multicenter outcome studies in patients with thrombocytopenia.
KW - bleeding
KW - blood platelets
KW - flow cytometry
KW - platelet activation
KW - thrombocytopenia
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U2 - 10.1016/j.jtha.2023.07.006
DO - 10.1016/j.jtha.2023.07.006
M3 - Article
C2 - 37481072
AN - SCOPUS:85167832498
SN - 1538-7933
VL - 21
SP - 2941
EP - 2952
JO - Journal of Thrombosis and Haemostasis
JF - Journal of Thrombosis and Haemostasis
IS - 10
ER -