TY - JOUR
T1 - Comparison of in vivo and in vitro binding of polycyclic hydrocarbons to DNA
AU - Eastman, Alan
AU - Sweetenham, John
AU - Bresnick, Edward
N1 - Funding Information:
We wish to thank Dr. R. Kimbrough for the sample of Arochlor 1254. This work was supported by a grant from the American Cancer Society, ACS BC 188 and from the NIH (CA23514).
PY - 1978/12
Y1 - 1978/12
N2 - The in vivo binding of [3H]benzo(a)pyrene (BP) and 3-[3H]methylcholanthrene (3MC) to liver and lung DNA was studied in A/J mice. Only in liver was there any reduction in total DNA-bound radioactivity between 4 h and 24 h after administration of the hydrocarbon. DNA was fractionated on Sephadex LH-20 after enzymatic digestion. A single deoxyribonucleoside-BP adduct was detected whereas two major 3MC-adducts were observed. With both BP and 3MC, three additional peaks of radioactivity eluted rapidly in the lung DNA experiments while a fourth was noted with liver DNA. The nucleoside-bound adducts from lung represented a much larger proportion of the total radioactivity than with liver. In vitro analysis of 3MC binding to DNA showed the nucleoside-bound adducts to be predominantly deoxyguanosine-dependent but that the early peaks were independent of base suggesting binding to another part of the DNA molecule, perhaps phosphate, i.e., phosphotriesters.
AB - The in vivo binding of [3H]benzo(a)pyrene (BP) and 3-[3H]methylcholanthrene (3MC) to liver and lung DNA was studied in A/J mice. Only in liver was there any reduction in total DNA-bound radioactivity between 4 h and 24 h after administration of the hydrocarbon. DNA was fractionated on Sephadex LH-20 after enzymatic digestion. A single deoxyribonucleoside-BP adduct was detected whereas two major 3MC-adducts were observed. With both BP and 3MC, three additional peaks of radioactivity eluted rapidly in the lung DNA experiments while a fourth was noted with liver DNA. The nucleoside-bound adducts from lung represented a much larger proportion of the total radioactivity than with liver. In vitro analysis of 3MC binding to DNA showed the nucleoside-bound adducts to be predominantly deoxyguanosine-dependent but that the early peaks were independent of base suggesting binding to another part of the DNA molecule, perhaps phosphate, i.e., phosphotriesters.
UR - http://www.scopus.com/inward/record.url?scp=0018256706&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018256706&partnerID=8YFLogxK
U2 - 10.1016/0009-2797(78)90095-9
DO - 10.1016/0009-2797(78)90095-9
M3 - Article
C2 - 719814
AN - SCOPUS:0018256706
SN - 0009-2797
VL - 23
SP - 345
EP - 353
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
IS - 3
ER -