Comparative Transcriptomics of Ex Vivo, Patient-Derived Endothelial Cells Reveals Novel Pathways Associated With Type 2 Diabetes Mellitus

Joshua A. Beckman; Sean P. Doherty; Zachary B. Feldman; Emily S. Banks; Javid Moslehi; Iris Z. Jaffe; Naomi M. Hamburg; Quanhu Sheng; Jonathan D. Brown

doi:10.1016/j.jacbts.2019.05.012

Comparative Transcriptomics of Ex Vivo, Patient-Derived Endothelial Cells Reveals Novel Pathways Associated With Type 2 Diabetes Mellitus

Joshua A. Beckman, Sean P. Doherty, Zachary B. Feldman, Emily S. Banks, Javid Moslehi, Iris Z. Jaffe, Naomi M. Hamburg, Quanhu Sheng, Jonathan D. Brown

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

In this study low-input RNA-sequencing was used to annotate the molecular identity of endothelial cells isolated and immunopurified with CD144 microbeads. Using this technique, comparative gene expression profiling from healthy subjects and patients with type 2 diabetes mellitus identified both known and novel pathways linked with EC dysfunction. Modeling of diabetes by treating cultured ECs with high glucose identified shared changes in gene expression in diabetic cells. Overall, the data demonstrate how purified ECs from patients can be used to generate new hypotheses about mechanisms of human vascular disease.

Original language	English (US)
Pages (from-to)	567-574
Number of pages	8
Journal	JACC: Basic to Translational Science
Volume	4
Issue number	5
DOIs	https://doi.org/10.1016/j.jacbts.2019.05.012
State	Published - Sep 2019
Externally published	Yes

Keywords

diabetes mellitus
endothelial cell dysfunction
endothelial cells
gene expression

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine

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10.1016/j.jacbts.2019.05.012

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Beckman, J. A., Doherty, S. P., Feldman, Z. B., Banks, E. S., Moslehi, J., Jaffe, I. Z., Hamburg, N. M., Sheng, Q., & Brown, J. D. (2019). Comparative Transcriptomics of Ex Vivo, Patient-Derived Endothelial Cells Reveals Novel Pathways Associated With Type 2 Diabetes Mellitus. JACC: Basic to Translational Science, 4(5), 567-574. https://doi.org/10.1016/j.jacbts.2019.05.012

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abstract = "In this study low-input RNA-sequencing was used to annotate the molecular identity of endothelial cells isolated and immunopurified with CD144 microbeads. Using this technique, comparative gene expression profiling from healthy subjects and patients with type 2 diabetes mellitus identified both known and novel pathways linked with EC dysfunction. Modeling of diabetes by treating cultured ECs with high glucose identified shared changes in gene expression in diabetic cells. Overall, the data demonstrate how purified ECs from patients can be used to generate new hypotheses about mechanisms of human vascular disease.",

keywords = "diabetes mellitus, endothelial cell dysfunction, endothelial cells, gene expression",

author = "Beckman, {Joshua A.} and Doherty, {Sean P.} and Feldman, {Zachary B.} and Banks, {Emily S.} and Javid Moslehi and Jaffe, {Iris Z.} and Hamburg, {Naomi M.} and Quanhu Sheng and Brown, {Jonathan D.}",

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year = "2019",

month = sep,

doi = "10.1016/j.jacbts.2019.05.012",

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AU - Beckman, Joshua A.

AU - Doherty, Sean P.

AU - Feldman, Zachary B.

AU - Banks, Emily S.

AU - Moslehi, Javid

AU - Jaffe, Iris Z.

AU - Hamburg, Naomi M.

AU - Sheng, Quanhu

AU - Brown, Jonathan D.

PY - 2019/9

Y1 - 2019/9

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KW - diabetes mellitus

KW - endothelial cell dysfunction

KW - endothelial cells

KW - gene expression

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Comparative Transcriptomics of Ex Vivo, Patient-Derived Endothelial Cells Reveals Novel Pathways Associated With Type 2 Diabetes Mellitus

Abstract

Keywords

ASJC Scopus subject areas

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