TY - JOUR
T1 - Common Genomic Response in Different Mouse Models of β -Adrenergic-Induced Cardiomyopathy
AU - Gaussin, Vinciane
AU - Tomlinson, James E.
AU - Depre, Christophe
AU - Engelhardt, Stefan
AU - Antos, Christopher L.
AU - Takagi, Gen
AU - Hein, Lutz
AU - Topper, James N.
AU - Liggett, Stephen B.
AU - Olson, Eric N.
AU - Lohse, Martin J.
AU - Vatner, Stephen F.
AU - Vatner, Dorothy E.
PY - 2003/12/9
Y1 - 2003/12/9
N2 - Background-Although β-adrenergic receptor (AR) blockade therapy is beneficial in the treatment of heart failure, little is known regarding the transcriptional mechanisms underlying this salutary action. Methods and Results-In the present study, we screened mice overexpressing Gsα, β1AR, β2AR, or protein kinase A to test if a common genomic pathway exists in different models with enhanced β-adrenergic signaling. In mice overexpressing Gsα, differentially expressed genes were identified by mRNA profiling. In addition to well-known markers of cardiac hypertrophy (atrial natriuretic factor, CARP, and β-myosin heavy chain), uncoupling protein 2 (UCP2), a protein involved in the control of mitochondrial membrane potential, and four-and-a-half LIM domain protein-1 (FHL1), a member of the LIM protein family, were predicted to be upregulated. Upregulation of these genes was confirmed by quantitative reverse transcriptase-polymerase chain reaction at all time points tested during the development of cardiomyopathy in mice overexpressing Gsα. In mice overexpressing β1AR, β2AR, or protein kinase A, increased UCP2 and FHL1 expression was also observed at the onset of cardiomyopathy. βAR blockade treatment reversed the cardiomyopathy and suppressed the increased expression of UCP2 and FHL1 in mice overexpressing Gsα. Conclusions-UCP2 and FHL1 are important candidate genes that correlate with the development of βAR-induced cardiomyopathy in different mouse models with enhanced βAR signaling. In addition to preserving cardiac function, βAR blockade treatment also prevents the genomic regulation that correlates with the onset of heart failure.
AB - Background-Although β-adrenergic receptor (AR) blockade therapy is beneficial in the treatment of heart failure, little is known regarding the transcriptional mechanisms underlying this salutary action. Methods and Results-In the present study, we screened mice overexpressing Gsα, β1AR, β2AR, or protein kinase A to test if a common genomic pathway exists in different models with enhanced β-adrenergic signaling. In mice overexpressing Gsα, differentially expressed genes were identified by mRNA profiling. In addition to well-known markers of cardiac hypertrophy (atrial natriuretic factor, CARP, and β-myosin heavy chain), uncoupling protein 2 (UCP2), a protein involved in the control of mitochondrial membrane potential, and four-and-a-half LIM domain protein-1 (FHL1), a member of the LIM protein family, were predicted to be upregulated. Upregulation of these genes was confirmed by quantitative reverse transcriptase-polymerase chain reaction at all time points tested during the development of cardiomyopathy in mice overexpressing Gsα. In mice overexpressing β1AR, β2AR, or protein kinase A, increased UCP2 and FHL1 expression was also observed at the onset of cardiomyopathy. βAR blockade treatment reversed the cardiomyopathy and suppressed the increased expression of UCP2 and FHL1 in mice overexpressing Gsα. Conclusions-UCP2 and FHL1 are important candidate genes that correlate with the development of βAR-induced cardiomyopathy in different mouse models with enhanced βAR signaling. In addition to preserving cardiac function, βAR blockade treatment also prevents the genomic regulation that correlates with the onset of heart failure.
KW - Cardiomyopathy
KW - Genomics
KW - Receptors, adrenergic, beta
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U2 - 10.1161/01.CIR.0000101922.18151.7B
DO - 10.1161/01.CIR.0000101922.18151.7B
M3 - Article
C2 - 14623810
AN - SCOPUS:0345167075
SN - 0009-7322
VL - 108
SP - 2926
EP - 2933
JO - Circulation
JF - Circulation
IS - 23
ER -