TY - JOUR
T1 - Coated-vesicle formation in vitro
T2 - conflicting results using different assays
AU - Schmid, Sandra L.
N1 - Funding Information:
I thank members of my laboratory and W. E. Balch for helpful dis cussions and con structive criticism. Research in my laboratory has been supported by the NIH and by the Lucille P. Markey Charitable Trust in the form of a Lucille P. Markey Scholarship. This is TSRI manuscript no. 7735-CB.
PY - 1993/5
Y1 - 1993/5
N2 - Cell-free systems provide essential tools for elucidating the molecular mechanisms underlying complex cellular processes such as vesicular transport. The biochemical utility of these model systems is strengthened by assays that allow rapid, quantitative detection of the events being studied. Two model systems have recently been developed to reconstitute coated-vesicle budding, and two different biochemical assays are used to detect this event. Striking differences in the biochemical requirements for 'coated-vesicle budding' are detected by these two assays, suggesting that two distinct events are being measured. These findings have wide implications for the use of cell-free assay systems in cell biology.
AB - Cell-free systems provide essential tools for elucidating the molecular mechanisms underlying complex cellular processes such as vesicular transport. The biochemical utility of these model systems is strengthened by assays that allow rapid, quantitative detection of the events being studied. Two model systems have recently been developed to reconstitute coated-vesicle budding, and two different biochemical assays are used to detect this event. Striking differences in the biochemical requirements for 'coated-vesicle budding' are detected by these two assays, suggesting that two distinct events are being measured. These findings have wide implications for the use of cell-free assay systems in cell biology.
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U2 - 10.1016/0962-8924(93)90129-O
DO - 10.1016/0962-8924(93)90129-O
M3 - Comment/debate
C2 - 14731608
AN - SCOPUS:0027406045
SN - 0962-8924
VL - 3
SP - 145
EP - 148
JO - Trends in Cell Biology
JF - Trends in Cell Biology
IS - 5
ER -