TY - JOUR
T1 - Co-stimulation with TNF receptor superfamily 4/25 antibodies enhances in-vivo expansion of CD4+CD25+Foxp3+ T cells (Tregs) in a mouse study for active DNA Aβ42 immunotherapy
AU - Lambracht-Washington, Doris
AU - Rosenberg, Roger N.
N1 - Funding Information:
This study was funded by grants from the NIH/NIA to the Alzheimer's Disease Center ( P30AG12300-19 ), the Rudman Partnership; Darryl Royal Foundation and McCune Foundation to RNR and an RO3 grant to DLW ( R03AG042697-02 ).
Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/1/15
Y1 - 2015/1/15
N2 - The study was designed to test DNA Aβ42 immunization in mice as alternative approach for possible active immunotherapy in Alzheimer patients. As results, we found polarized Th2 immune responses, efficient Aβ42 antibody levels, and disappearance of antigen specific T cells. In-vivo TNFRSF4/25 antibody co-stimulation enhanced Aβ42 specific T cell responses with initial Th2 expansion and subsequent development of Aβ42 specific CD4. +. CD25. +. Foxp3. + cells. It showed that Th2 biased responses due to gene gun immunizations propagate the development of regulatory T cells. In conclusion, full-length DNA Aβ42 immunization into skin results in a regulatory response with minimal risk of inflammation and autoimmunity.
AB - The study was designed to test DNA Aβ42 immunization in mice as alternative approach for possible active immunotherapy in Alzheimer patients. As results, we found polarized Th2 immune responses, efficient Aβ42 antibody levels, and disappearance of antigen specific T cells. In-vivo TNFRSF4/25 antibody co-stimulation enhanced Aβ42 specific T cell responses with initial Th2 expansion and subsequent development of Aβ42 specific CD4. +. CD25. +. Foxp3. + cells. It showed that Th2 biased responses due to gene gun immunizations propagate the development of regulatory T cells. In conclusion, full-length DNA Aβ42 immunization into skin results in a regulatory response with minimal risk of inflammation and autoimmunity.
KW - Alzheimer disease
KW - DNA Aβ42 immunization
KW - Skin immunity
KW - TNFRSF4/25 antibody co-stimulation
KW - Th2/Treg differentiation
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U2 - 10.1016/j.jneuroim.2014.12.007
DO - 10.1016/j.jneuroim.2014.12.007
M3 - Article
C2 - 25595257
AN - SCOPUS:84920911203
SN - 0165-5728
VL - 278
SP - 90
EP - 99
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
ER -