TY - JOUR
T1 - Circulating glucose levels inversely correlate with Drosophila larval feeding through insulin signaling and SLC5A11
AU - Ugrankar, Rupali
AU - Theodoropoulos, Pano
AU - Akdemir, Fatih
AU - Henne, W. Mike
AU - Graff, Jonathan M.
N1 - Funding Information:
We are grateful to David Mangelsdorf and Steven Kliewer for use of equipment. We thank the Bloomington Stock Center for fly stocks. We are also grateful to members of the Graff and Henne labs for support, reagents, and insights. This study was supported by the National Institute of Health and the National Institute of Diabetes and Digestive
Funding Information:
and Kidney Disease grants (R01 DK066556, R01 DK064261, and R01 DK088220) to J.M. G., and by the American Diabetes Association grant (7-12-MN-23) to R.U and J.M.G.
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - In mammals, blood glucose levels likely play a role in appetite regulation yet the mechanisms underlying this phenomenon remain opaque. Mechanisms can often be explored from Drosophila genetic approaches. To determine if circulating sugars might be involved in Drosophila feeding behaviors, we scored hemolymph glucose and trehalose, and food ingestion in larvae subjected to various diets, genetic mutations, or RNAi. We found that larvae with glucose elevations, hyperglycemia, have an aversion to feeding; however, trehalose levels do not track with feeding behavior. We further discovered that insulins and SLC5A11 may participate in glucose-regulated feeding. To see if food aversion might be an appropriate screening method for hyperglycemia candidates, we developed a food aversion screen to score larvae with abnormal feeding for glucose. We found that many feeding defective larvae have glucose elevations. These findings highlight intriguing roles for glucose in fly biology as a potential cue and regulator of appetite.
AB - In mammals, blood glucose levels likely play a role in appetite regulation yet the mechanisms underlying this phenomenon remain opaque. Mechanisms can often be explored from Drosophila genetic approaches. To determine if circulating sugars might be involved in Drosophila feeding behaviors, we scored hemolymph glucose and trehalose, and food ingestion in larvae subjected to various diets, genetic mutations, or RNAi. We found that larvae with glucose elevations, hyperglycemia, have an aversion to feeding; however, trehalose levels do not track with feeding behavior. We further discovered that insulins and SLC5A11 may participate in glucose-regulated feeding. To see if food aversion might be an appropriate screening method for hyperglycemia candidates, we developed a food aversion screen to score larvae with abnormal feeding for glucose. We found that many feeding defective larvae have glucose elevations. These findings highlight intriguing roles for glucose in fly biology as a potential cue and regulator of appetite.
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U2 - 10.1038/s42003-018-0109-4
DO - 10.1038/s42003-018-0109-4
M3 - Article
C2 - 30271990
AN - SCOPUS:85071155913
SN - 2399-3642
VL - 1
JO - Communications Biology
JF - Communications Biology
IS - 1
M1 - 110
ER -