TY - JOUR
T1 - Chitosan-silver nanoparticles as an approach to control bacterial proliferation, spores and antibiotic-resistant bacteria
AU - Alfaro-González, B.
AU - Ulate, D.
AU - Alvarado, R.
AU - Arguëllo-Miranda, O.
N1 - Funding Information:
This work was supported by the National Center for Advanced Technologies and the National Laboratory of Nanotechnology. We thank Elizabeth Argüello for image editing and Rocío Zamora for fruitful discussions regarding the chitosan chemistry in alkaline media.
Publisher Copyright:
© 2018 IOP Publishing Ltd.
PY - 2018/3/9
Y1 - 2018/3/9
N2 - Nanoparticulate silver inhibits bacterial growth. Here we evaluated the antimicrobial properties of a novel form of silver nanoparticles, synthesized by a sonochemical method with chitosan as reducing and stabilizing agent (chitosan-silver nanoparticles). The chitosan-silver nanoparticles were positively charged and stable in the long-term, as determined by the high Z-potential. The minimum inhibitory concentration for vegetative bacteria and spores was measured with a resazurin microtiter assay. The interaction between nanoparticles and the bacterial surface was observed by atomic force microscopy and transmission electron microscopy. We found that chitosan-silver nanoparticles inhibited vegetative bacteria and bacterial spores. Furthermore, chitosan-silver nanoparticles enhanced the performance of ampicillin against bacteria and were also effective against an ampicillin-resistant strain. Atomic force microscopy and transmission electron microscopy images revealed a close interaction between the nanoparticles and bacterial extracellular structures, such as flagella. We suggest that chitosan-silver nanoparticles could be used as a topical antimicrobial that can enhance antibiotic performance.
AB - Nanoparticulate silver inhibits bacterial growth. Here we evaluated the antimicrobial properties of a novel form of silver nanoparticles, synthesized by a sonochemical method with chitosan as reducing and stabilizing agent (chitosan-silver nanoparticles). The chitosan-silver nanoparticles were positively charged and stable in the long-term, as determined by the high Z-potential. The minimum inhibitory concentration for vegetative bacteria and spores was measured with a resazurin microtiter assay. The interaction between nanoparticles and the bacterial surface was observed by atomic force microscopy and transmission electron microscopy. We found that chitosan-silver nanoparticles inhibited vegetative bacteria and bacterial spores. Furthermore, chitosan-silver nanoparticles enhanced the performance of ampicillin against bacteria and were also effective against an ampicillin-resistant strain. Atomic force microscopy and transmission electron microscopy images revealed a close interaction between the nanoparticles and bacterial extracellular structures, such as flagella. We suggest that chitosan-silver nanoparticles could be used as a topical antimicrobial that can enhance antibiotic performance.
KW - AFM
KW - TEM,REMA
KW - antibiotic resistance
KW - chitosan-silver nanoparticles
KW - flagella
KW - sonochemical synthesis
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U2 - 10.1088/2057-1976/aaaafe
DO - 10.1088/2057-1976/aaaafe
M3 - Article
AN - SCOPUS:85047244018
SN - 2057-1976
VL - 4
JO - Biomedical Physics and Engineering Express
JF - Biomedical Physics and Engineering Express
IS - 3
M1 - 035011
ER -