@article{82506c796ad5496c90ebd5d3b1dca738,
title = "Chemical and structural analysis of a photoactive vertebrate cryptochrome from pigeon",
abstract = "Computational and biochemical studies implicate the blue-light sensor cryptochrome (CRY) as an endogenous light-dependent magnetosensor enabling migratory birds to navigate using the Earth's magnetic field. Validation of such a mechanism has been hampered by the absence of structures of vertebrate CRYs that have functional photochemistry. Here we present crystal structures of Columba livia (pigeon) CRY4 that reveal evolutionarily conserved modifications to a sequence of Trp residues (Trp-triad) required for CRY photoreduction. In ClCRY4, the Trp-triad chain is extended to include a fourth Trp (W369) and a Tyr (Y319) residue at the protein surface that imparts an unusually high quantum yield of photoreduction. These results are consistent with observations of night migratory behavior in animals at low light levels and could have implications for photochemical pathways allowing magnetosensing.",
keywords = "Cryptochromes, Magnetoreception, Photobiology",
author = "Zoltowski, {Brian D.} and Yogarany Chelliah and Anushka Wickramaratne and Lauren Jarocha and Nischal Karki and Wei Xu and Henrik Mouritsen and Hore, {Peter J.} and Hibbs, {Ryan E.} and Green, {Carla B.} and Takahashi, {Joseph S.}",
note = "Funding Information: R35 GM127122, to C.B.G.), Deutsche Forschungsgemeinschaft (SFB 1372, “Magnetoreception and Navigation in Vertebrates” and GRK 1885, to H.M.), the Air Force Office of Scientific Research (Air Force Materiel Command, USAF Award FA9550-14-1-0095, to P.J.H. and H.M.), the European Research Council (under the European Union{\textquoteright}s 7th Framework Programme, FP7/2007-2013/ERC Grant Agreement 340451, to P.J.H. and under the Horizon 2020 research and innovation program, ERC Synergy Grant Agreement 810002, to P.J.H. and H.M.). X-ray diffraction experiments on WT protein were performed at the Northeastern Collaborative Access Team beamlines, which are funded by the NIH, National Institute of General Medical Sciences (P30 GM124165). The Pilatus 6M detector on the 24-ID-C beamline is funded by NIH Office of Research Infrastructure Programs High-End Instrumentation Grant S10 RR029205. This research used resources of the Advanced Photon Source, a US Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract DE-AC02-06CH11357. The Berkeley Center for Structural Biology is supported in part by the NIH, National Institute of General Medical Sciences, and the HHMI. X-ray diffraction experiments on Y319D mutant protein were conducted on the 8.2.1 (HHMI) beamline at the Advanced Light Source. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences of the US Department of Energy under Contract DE-AC02-05CH11231. J.S.T. is an HHMI Investigator. Funding Information: ACKNOWLEDGMENTS. We thank the beamline staff of the Advanced Light Source at the University of California at Berkeley for help with data collection. This work was funded by the NSF (MCB-1613643, to B.D.Z.), NIH (NS095899 and DA042072, to R.E.H.; R01 GM090247, R01 GM112991, and Funding Information: This work was funded by the NSF (MCB-1613643, to B.D.Z.), NIH (NS095899 and DA042072, to R.E.H.; R01 GM090247, R01 GM112991, and R35 GM127122, to C.B.G.), Deutsche Forschungsgemeinschaft (SFB 1372, Magnetoreception and Navigation in Vertebrates and GRK 1885, to H.M.), the Air Force Office of Scientific Research (Air Force Materiel Command, USAF Award FA9550-14-1-0095, to P.J.H. and H.M.), the European Research Council (under the European Union's 7th Framework Programme, FP7/2007- 2013/ERC Grant Agreement 340451, to P.J.H. and under the Horizon 2020 research and innovation program, ERC Synergy Grant Agreement 810002, to P.J.H. and H.M.). X-ray diffraction experiments on WT protein were performed at the Northeastern Collaborative Access Team beamlines, which are funded by the NIH, National Institute of General Medical Sciences (P30 GM124165). The Pilatus 6M detector on the 24-ID-C beamline is funded by NIH Office of Research Infrastructure Programs High-End Instrumentation Grant S10 RR029205. This research used resources of the Advanced Photon Source, a US Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract DE-AC02-06CH11357. The Berkeley Center for Structural Biology is supported in part by the NIH, National Institute of General Medical Sciences, and the HHMI. X-ray diffraction experiments on Y319D mutant protein were conducted on the 8.2.1 (HHMI) beamline at the Advanced Light Source. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences of the US Department of Energy under Contract DE-AC02-05CH11231. J.S.T. is an HHMI Investigator Publisher Copyright: {\textcopyright} 2019 National Academy of Sciences. All rights reserved.",
year = "2019",
month = sep,
day = "24",
doi = "10.1073/pnas.1907875116",
language = "English (US)",
volume = "116",
pages = "19449--19457",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "39",
}