Characterization of immunoreactive α-melanocyte stimulating hormone (α-MSHi) in human brain tissue

C. Richard Parker; Ayalla Barnea; Fred J H Tilders; John C. Porter

doi:10.1016/S0361-9230(81)80058-5

Characterization of immunoreactive α-melanocyte stimulating hormone (α-MSH_i) in human brain tissue

C. Richard Parker, Ayalla Barnea, Fred J H Tilders, John C. Porter

Obstetrics & Gynecology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Adult human hypothalamic tissue was analyzed for the presence of products generated by post-cleavage processing of ACTH 1-13. The displacement curve generated by immunoreactive a-melanocyte stimulating hormone (α-MSH_i) in extracts (5 M acetic acid or acidified ethanol) of adult human hypothalamic tissue was parallel to the α-MSH radioimmunoassay standard curve, and α-MSH₁ eluted as a single peak on Sephadex G-10, G-25, and G-50 columns in a manner identical to that of synthetic α-MSH₁. The α-MSH₁ was clearly distinguishable in its mobility on Sephadex G-50 columns from such peptides as α-MSH, luteinizing hormone releasing hormone, and ACTH. After high performance liquid chromatography of extracts of hypothalamic tissue from young as well as aged men and women, we found that the major peak of α-MSH₁ (75-95% of total α-MSH₁) coeluted with desacetyl α-MSH₁(ACTH 1-13 amide) rather than α-MSH. We suggest that desacetyl α-MSH₁, rather than α-MSH₁, is the predominant α-MSH₁ in adult human brain tissue.

Original language	English (US)
Pages (from-to)	275-280
Number of pages	6
Journal	Brain Research Bulletin
Volume	6
Issue number	3
DOIs	https://doi.org/10.1016/S0361-9230(81)80058-5
State	Published - Mar 1981

Keywords

ACTH
ACTH 1-13 amide
Adrenocorticotropic hormone
Desacetyl α-MSH
High performance liquid chromatography
Human brain
Melanocyte stimulating hormone
α-MSH

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/S0361-9230(81)80058-5

Cite this

@article{00b65dba205742519b1753239b2e89d9,

title = "Characterization of immunoreactive α-melanocyte stimulating hormone (α-MSHi) in human brain tissue",

abstract = "Adult human hypothalamic tissue was analyzed for the presence of products generated by post-cleavage processing of ACTH 1-13. The displacement curve generated by immunoreactive a-melanocyte stimulating hormone (α-MSHi) in extracts (5 M acetic acid or acidified ethanol) of adult human hypothalamic tissue was parallel to the α-MSH radioimmunoassay standard curve, and α-MSH1 eluted as a single peak on Sephadex G-10, G-25, and G-50 columns in a manner identical to that of synthetic α-MSH1. The α-MSH1 was clearly distinguishable in its mobility on Sephadex G-50 columns from such peptides as α-MSH, luteinizing hormone releasing hormone, and ACTH. After high performance liquid chromatography of extracts of hypothalamic tissue from young as well as aged men and women, we found that the major peak of α-MSH1 (75-95% of total α-MSH1) coeluted with desacetyl α-MSH1(ACTH 1-13 amide) rather than α-MSH. We suggest that desacetyl α-MSH1, rather than α-MSH1, is the predominant α-MSH1 in adult human brain tissue.",

keywords = "ACTH, ACTH 1-13 amide, Adrenocorticotropic hormone, Desacetyl α-MSH, High performance liquid chromatography, Human brain, Melanocyte stimulating hormone, α-MSH",

author = "Parker, {C. Richard} and Ayalla Barnea and Tilders, {Fred J H} and Porter, {John C.}",

note = "Funding Information: Brain tissue of 27 adult humans (23-65 years of age) was dissected at autopsy as described previously \[12\].T he tissue was homogenized at 4°C in 10 volumes of ice-cold acidified ethanol (75:15:10; ethanol:water:l M acetic acid, v/v). The homogenates were centrifuged at 3,000×g for 10 min at 4°C, and the supernatant fluid was evaporated under a stream of ~Supported in part by research grants AG00306 and'AG02295 from the National Institutes of Health, Bethesda, MD. '-'Address all correspondence to: C. Richard Parker, Jr., Department of Obstetrics and Gynecology, University of Texas Health Science Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75235. '~Current address: Department of Pharmacology, Free University, Medical Faculty, Amsterdam, The Netherlands.",

year = "1981",

month = mar,

doi = "10.1016/S0361-9230(81)80058-5",

language = "English (US)",

volume = "6",

pages = "275--280",

journal = "Brain Research Bulletin",

issn = "0361-9230",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Characterization of immunoreactive α-melanocyte stimulating hormone (α-MSHi) in human brain tissue

AU - Parker, C. Richard

AU - Barnea, Ayalla

AU - Tilders, Fred J H

AU - Porter, John C.

N1 - Funding Information: Brain tissue of 27 adult humans (23-65 years of age) was dissected at autopsy as described previously \[12\].T he tissue was homogenized at 4°C in 10 volumes of ice-cold acidified ethanol (75:15:10; ethanol:water:l M acetic acid, v/v). The homogenates were centrifuged at 3,000×g for 10 min at 4°C, and the supernatant fluid was evaporated under a stream of ~Supported in part by research grants AG00306 and'AG02295 from the National Institutes of Health, Bethesda, MD. '-'Address all correspondence to: C. Richard Parker, Jr., Department of Obstetrics and Gynecology, University of Texas Health Science Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75235. '~Current address: Department of Pharmacology, Free University, Medical Faculty, Amsterdam, The Netherlands.

PY - 1981/3

Y1 - 1981/3

N2 - Adult human hypothalamic tissue was analyzed for the presence of products generated by post-cleavage processing of ACTH 1-13. The displacement curve generated by immunoreactive a-melanocyte stimulating hormone (α-MSHi) in extracts (5 M acetic acid or acidified ethanol) of adult human hypothalamic tissue was parallel to the α-MSH radioimmunoassay standard curve, and α-MSH1 eluted as a single peak on Sephadex G-10, G-25, and G-50 columns in a manner identical to that of synthetic α-MSH1. The α-MSH1 was clearly distinguishable in its mobility on Sephadex G-50 columns from such peptides as α-MSH, luteinizing hormone releasing hormone, and ACTH. After high performance liquid chromatography of extracts of hypothalamic tissue from young as well as aged men and women, we found that the major peak of α-MSH1 (75-95% of total α-MSH1) coeluted with desacetyl α-MSH1(ACTH 1-13 amide) rather than α-MSH. We suggest that desacetyl α-MSH1, rather than α-MSH1, is the predominant α-MSH1 in adult human brain tissue.

AB - Adult human hypothalamic tissue was analyzed for the presence of products generated by post-cleavage processing of ACTH 1-13. The displacement curve generated by immunoreactive a-melanocyte stimulating hormone (α-MSHi) in extracts (5 M acetic acid or acidified ethanol) of adult human hypothalamic tissue was parallel to the α-MSH radioimmunoassay standard curve, and α-MSH1 eluted as a single peak on Sephadex G-10, G-25, and G-50 columns in a manner identical to that of synthetic α-MSH1. The α-MSH1 was clearly distinguishable in its mobility on Sephadex G-50 columns from such peptides as α-MSH, luteinizing hormone releasing hormone, and ACTH. After high performance liquid chromatography of extracts of hypothalamic tissue from young as well as aged men and women, we found that the major peak of α-MSH1 (75-95% of total α-MSH1) coeluted with desacetyl α-MSH1(ACTH 1-13 amide) rather than α-MSH. We suggest that desacetyl α-MSH1, rather than α-MSH1, is the predominant α-MSH1 in adult human brain tissue.

KW - ACTH

KW - ACTH 1-13 amide

KW - Adrenocorticotropic hormone

KW - Desacetyl α-MSH

KW - High performance liquid chromatography

KW - Human brain

KW - Melanocyte stimulating hormone

KW - α-MSH

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U2 - 10.1016/S0361-9230(81)80058-5

DO - 10.1016/S0361-9230(81)80058-5

M3 - Article

C2 - 6261892

AN - SCOPUS:0019796544

SN - 0361-9230

VL - 6

SP - 275

EP - 280

JO - Brain Research Bulletin

JF - Brain Research Bulletin

IS - 3

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