Changes in the protein pattern of murine B cells after mitogenic stimulation

J. Kettman, I. Lefkovits

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The technique of two‐dimensional gel electrophoresis has been applied to study the changes in protein patterns occurring in murine B lymphocytes after activation with the synergistic B cell mitogens lipopolysaccharide and dextran sulfate. Whole cell lysates from [35S]methionine‐labeled mitogen‐activated B cells were analyzed and radiofluorographs of the gels compared. We found that a large number of proteins appear and disappear during the 5‐day activation period (74/206 spots of a nonimmunoglobulin region of the gel). The B cell pattern (in the nonimmunoglobulin region) was also compared with that of mitogen‐activated T cells. We identified 19 T cell‐specific and 29 B cell‐specific proteins, the remaining 1000 or so proteins being common.

Original languageEnglish (US)
Pages (from-to)778-781
Number of pages4
JournalEuropean Journal of Immunology
Issue number9
StatePublished - 1984

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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