Cerebral microvessel endothelium is producing endothelin

Satonobu Yoshimoto, Yasuki Ishizaki, Hiroki Kurihara, Tomio Sasaki, Masao Yoshizumi, Masashi Yanagisawa, Yoshio Yazaki, Tomoh Masaki, Kintomo Takakura, Sei itsu Murota

Research output: Contribution to journalArticlepeer-review

120 Scopus citations


Endothelin, a potent vasoconstrictor peptide, was recently isolated from the supernatant of the cultured endothelia of the porcine aorta and is now supposed to be the most likely candidate for the endothelium-derived contractile factor (EDCF), which is responsible for the endothelium-dependent vasoconstriction by various stimuli. In this study, the production of endothelin by the endothelia of porcine cerebral microvessels was revealed by the Northern blot analysis with porcine endothelin cDNA probe and the enzyme-linked immunosorbent assay (ELISA) with anti-porcine endothelin antibody. Our results raise the possibility that the endothelia of cerebral microvessels regulate the local blood flow within the brain through the production of endothelin.

Original languageEnglish (US)
Pages (from-to)283-285
Number of pages3
JournalBrain Research
Issue number2
StatePublished - Feb 5 1990


  • Brain microvessel
  • Cell culture
  • Endothelial cell
  • Endothelin
  • Northern analysis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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