TY - JOUR
T1 - Cdc14 spatiotemporally dephosphorylates Atg13 to activate autophagy during meiotic divisions
AU - Feng, Wenzhi
AU - Argüello-Miranda, Orlando
AU - Qian, Suhong
AU - Wang, Fei
N1 - Funding Information:
This work was supported by a grant from the National Institutes of Health to F. Wang (R01GM133899) and from the Welch Foundation to F. Wang (I-2019-20190330), as well as funding from Nancy Cain and Jeffrey A. Marcus Scholar in Medical Research, in Honor of Dr. Bill S. Vowell, to F. Wang, and the National Institute of General Medical Sciences of the National Institutes of Health (K99GM135487) to O. Argüello-Miranda. The authors declare no competing financial interests.
Funding Information:
We thank Beth Levine, the University of Texas Southwestern Medical Center for Autophagy Research and Benjamin Tu for imaging and experimental support. The authors would like to acknowledge the assistance of the University of Texas South-western Quantitative Light Microscopy Core, a Shared Resource of the Harold C. Simmons Cancer Center, supported in part by an NCI Cancer Center Support Grant, 1P30 CA142543-01, and an NIH Shared Instrumentation Award to Dr. K. Luby-Phelps (1S10OD028630-01). We thank Vladimir Denic (Harvard University, Boston, MA) and Soni Lacefield (Indiana University, Bloomington, IN) for reagents and protocols. We thank B. Levine, J. Seemann, J. Friedman, S. Lacefield, M. Henne, and members of the Wang lab for comments on the manuscript.
Publisher Copyright:
© 2022 Feng et al.
PY - 2022/5/2
Y1 - 2022/5/2
N2 - Autophagy is a conserved eukaryotic lysosomal degradation pathway that responds to environmental and cellular cues. Autophagy is essential for the meiotic exit and sporulation in budding yeast, but the underlying molecular mechanisms remain unknown. Here, we show that autophagy is maintained during meiosis and stimulated in anaphase I and II. Cells with higher levels of autophagy complete meiosis faster, and genetically enhanced autophagy increases meiotic kinetics and sporulation efficiency. Strikingly, our data reveal that the conserved phosphatase Cdc14 regulates meiosis-specific autophagy. Cdc14 is activated in anaphase I and II, accompanying its subcellular relocation from the nucleolus to the cytoplasm, where it dephosphorylates Atg13 to stimulate Atg1 kinase activity and thus autophagy. Together, our findings reveal a meiosis-tailored mechanism that spatiotemporally controls meiotic autophagy activity to ensure meiosis progression, exit, and sporulation.
AB - Autophagy is a conserved eukaryotic lysosomal degradation pathway that responds to environmental and cellular cues. Autophagy is essential for the meiotic exit and sporulation in budding yeast, but the underlying molecular mechanisms remain unknown. Here, we show that autophagy is maintained during meiosis and stimulated in anaphase I and II. Cells with higher levels of autophagy complete meiosis faster, and genetically enhanced autophagy increases meiotic kinetics and sporulation efficiency. Strikingly, our data reveal that the conserved phosphatase Cdc14 regulates meiosis-specific autophagy. Cdc14 is activated in anaphase I and II, accompanying its subcellular relocation from the nucleolus to the cytoplasm, where it dephosphorylates Atg13 to stimulate Atg1 kinase activity and thus autophagy. Together, our findings reveal a meiosis-tailored mechanism that spatiotemporally controls meiotic autophagy activity to ensure meiosis progression, exit, and sporulation.
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U2 - 10.1083/jcb.202107151
DO - 10.1083/jcb.202107151
M3 - Article
C2 - 35238874
AN - SCOPUS:85125614428
SN - 0021-9525
VL - 221
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
M1 - e202107151
ER -