Calcium modulation and high calcium permeability of neuronal nicotinic acetylcholine receptors

Steven Vernino; Mariano Amador; Charles W. Luetje; Jim Patrick; John A. Dani

doi:10.1016/0896-6273(92)90114-S

Calcium modulation and high calcium permeability of neuronal nicotinic acetylcholine receptors

Steven Vernino, Mariano Amador, Charles W. Luetje, Jim Patrick, John A. Dani

Research output: Contribution to journal › Article › peer-review

407 Scopus citations

Abstract

Two properties were found to distinguish neuronal from muscle nicotinic acetylcholine receptors (nAChRs). First, neuronal nAChRs have a greater Ca ²⁺ permeability. The high Ca²⁺ flux through neuronal nAChRs activates a Ca²⁺-dependent CI^- conductance, and the Ca ²⁺ to Cs⁺ permeability ratio ( P_Ca P_Cs) is 7 times greater for neuronal than for muscle nAChRs. A second difference between the receptor types is that neuronal nAChRs are potently modulated by physiological levels of external Ca ²⁺. Neuronal nAChR currents are enhanced by external Ca²⁺ in a dose-dependent manner. The results indicate that changes in extracellular Call modulate neuronal nAChRs and may modulate cholinergic synapses in the CNS. Also, activation of neuronal nAChRs produces a significant influx of Ca²⁺ that could be an important intracellular signal.

Original language	English (US)
Pages (from-to)	127-134
Number of pages	8
Journal	Neuron
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/0896-6273(92)90114-S
State	Published - Jan 1992

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/0896-6273(92)90114-S

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title = "Calcium modulation and high calcium permeability of neuronal nicotinic acetylcholine receptors",

abstract = "Two properties were found to distinguish neuronal from muscle nicotinic acetylcholine receptors (nAChRs). First, neuronal nAChRs have a greater Ca 2+ permeability. The high Ca2+ flux through neuronal nAChRs activates a Ca2+-dependent CI- conductance, and the Ca 2+ to Cs+ permeability ratio ( PCa PCs) is 7 times greater for neuronal than for muscle nAChRs. A second difference between the receptor types is that neuronal nAChRs are potently modulated by physiological levels of external Ca 2+. Neuronal nAChR currents are enhanced by external Ca2+ in a dose-dependent manner. The results indicate that changes in extracellular Call modulate neuronal nAChRs and may modulate cholinergic synapses in the CNS. Also, activation of neuronal nAChRs produces a significant influx of Ca2+ that could be an important intracellular signal.",

author = "Steven Vernino and Mariano Amador and Luetje, {Charles W.} and Jim Patrick and Dani, {John A.}",

note = "Funding Information: We thank G. Schuster, E. Millman, J. Redell, V. Hemelt, and Dr. 1. Waymire for their help in preparing bovine chromaffin cells and T. Afinni, K. Brousseau, and D. Chen for their help with tissue culture. We thank K. Flood and S. Zatechka for their help with Ca2+ injections into oocytes. This work was supported by the Muscular Dystrophy Association, by the Whitaker Foundation, and by the National Institutes of Health. C. W. L. was supported by a National Research Service Award fro{\textquoteright}m the National Institute of Mental Health.",

year = "1992",

month = jan,

doi = "10.1016/0896-6273(92)90114-S",

language = "English (US)",

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journal = "Neuron",

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AU - Vernino, Steven

AU - Amador, Mariano

AU - Luetje, Charles W.

AU - Patrick, Jim

AU - Dani, John A.

N1 - Funding Information: We thank G. Schuster, E. Millman, J. Redell, V. Hemelt, and Dr. 1. Waymire for their help in preparing bovine chromaffin cells and T. Afinni, K. Brousseau, and D. Chen for their help with tissue culture. We thank K. Flood and S. Zatechka for their help with Ca2+ injections into oocytes. This work was supported by the Muscular Dystrophy Association, by the Whitaker Foundation, and by the National Institutes of Health. C. W. L. was supported by a National Research Service Award fro’m the National Institute of Mental Health.

PY - 1992/1

Y1 - 1992/1

N2 - Two properties were found to distinguish neuronal from muscle nicotinic acetylcholine receptors (nAChRs). First, neuronal nAChRs have a greater Ca 2+ permeability. The high Ca2+ flux through neuronal nAChRs activates a Ca2+-dependent CI- conductance, and the Ca 2+ to Cs+ permeability ratio ( PCa PCs) is 7 times greater for neuronal than for muscle nAChRs. A second difference between the receptor types is that neuronal nAChRs are potently modulated by physiological levels of external Ca 2+. Neuronal nAChR currents are enhanced by external Ca2+ in a dose-dependent manner. The results indicate that changes in extracellular Call modulate neuronal nAChRs and may modulate cholinergic synapses in the CNS. Also, activation of neuronal nAChRs produces a significant influx of Ca2+ that could be an important intracellular signal.

AB - Two properties were found to distinguish neuronal from muscle nicotinic acetylcholine receptors (nAChRs). First, neuronal nAChRs have a greater Ca 2+ permeability. The high Ca2+ flux through neuronal nAChRs activates a Ca2+-dependent CI- conductance, and the Ca 2+ to Cs+ permeability ratio ( PCa PCs) is 7 times greater for neuronal than for muscle nAChRs. A second difference between the receptor types is that neuronal nAChRs are potently modulated by physiological levels of external Ca 2+. Neuronal nAChR currents are enhanced by external Ca2+ in a dose-dependent manner. The results indicate that changes in extracellular Call modulate neuronal nAChRs and may modulate cholinergic synapses in the CNS. Also, activation of neuronal nAChRs produces a significant influx of Ca2+ that could be an important intracellular signal.

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Calcium modulation and high calcium permeability of neuronal nicotinic acetylcholine receptors

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