Bovine blastocyst-like structures derived from stem cell cultures

Carlos A. Pinzón-Arteaga; Yinjuan Wang; Yulei Wei; Ana E. Ribeiro Orsi; Leijie Li; Giovanna Scatolin; Lizhong Liu; Masahiro Sakurai; Jianfeng Ye; Ming Hao Ming; Leqian Yu; Bo Li; Zongliang Jiang; Jun Wu

doi:10.1016/j.stem.2023.04.003

Bovine blastocyst-like structures derived from stem cell cultures

Carlos A. Pinzón-Arteaga, Yinjuan Wang, Yulei Wei, Ana E. Ribeiro Orsi, Leijie Li, Giovanna Scatolin, Lizhong Liu, Masahiro Sakurai, Jianfeng Ye, Ming Hao Ming, Leqian Yu, Bo Li, Zongliang Jiang, Jun Wu

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Understanding the mechanisms of blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. Here, we developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via assembling bovine trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, in vitro growth, and the ability to elicit maternal recognition of pregnancy following transfer to recipient cows. Bovine blastoids represent an accessible in vitro model for studying embryogenesis and improving reproductive efficiency in livestock species.

Original language	English (US)
Pages (from-to)	611-616.e7
Journal	Cell Stem Cell
Volume	30
Issue number	5
DOIs	https://doi.org/10.1016/j.stem.2023.04.003
State	Published - May 4 2023

Keywords

Bovine-blastocyst-like structures
blastoids
bovine blastoids
bovine embryonic stem cells
bovine expanded potential stem cells
bovine trophoblast stem cells

ASJC Scopus subject areas

Molecular Medicine
Genetics
Cell Biology

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10.1016/j.stem.2023.04.003

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title = "Bovine blastocyst-like structures derived from stem cell cultures",

abstract = "Understanding the mechanisms of blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. Here, we developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via assembling bovine trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, in vitro growth, and the ability to elicit maternal recognition of pregnancy following transfer to recipient cows. Bovine blastoids represent an accessible in vitro model for studying embryogenesis and improving reproductive efficiency in livestock species.",

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doi = "10.1016/j.stem.2023.04.003",

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AU - Pinzón-Arteaga, Carlos A.

AU - Wang, Yinjuan

AU - Wei, Yulei

AU - Ribeiro Orsi, Ana E.

AU - Li, Leijie

AU - Scatolin, Giovanna

AU - Liu, Lizhong

AU - Sakurai, Masahiro

AU - Ye, Jianfeng

AU - Hao Ming, Ming

AU - Yu, Leqian

AU - Li, Bo

AU - Jiang, Zongliang

AU - Wu, Jun

PY - 2023/5/4

Y1 - 2023/5/4

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AB - Understanding the mechanisms of blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. Here, we developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via assembling bovine trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, in vitro growth, and the ability to elicit maternal recognition of pregnancy following transfer to recipient cows. Bovine blastoids represent an accessible in vitro model for studying embryogenesis and improving reproductive efficiency in livestock species.

KW - Bovine-blastocyst-like structures

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KW - bovine blastoids

KW - bovine embryonic stem cells

KW - bovine expanded potential stem cells

KW - bovine trophoblast stem cells

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Bovine blastocyst-like structures derived from stem cell cultures

Abstract

Keywords

ASJC Scopus subject areas

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