TY - JOUR
T1 - Biologically synthesized silver nanoparticles induce neuronal differentiation of SH-SY5Y cells via modulation of reactive oxygen species, phosphatases, and kinase signaling pathways
AU - Dayem, Ahmed Abdal
AU - Kim, Bongwoo
AU - Gurunathan, Sangiliyandi
AU - Choi, Hye Yeon
AU - Yang, Gwangmo
AU - Saha, Subbroto Kumar
AU - Han, Dawoon
AU - Han, Jihae
AU - Kim, Kyeongseok
AU - Kim, Jin Hoi
AU - Cho, Ssang Goo
PY - 2014/7
Y1 - 2014/7
N2 - Nano-scale materials are noted for unique properties, distinct from those of their bulk material equivalents. In this study, we prepared spherical silver nanoparticles (AgNPs) with an average size of about 30 nm and tested their potency to induce neuronal differentiation of SH-SY5Y cells. Human neuroblastoma SH-SY5Y cells are considered an ideal in vitro model for studying neurogenesis, as they can be maintained in an undifferentiated state or be induced to differentiate into neuron-like phenotypes in vitro by several differentiation-inducing agents. Treatment of SH-SY5Y cells by biologically synthesized AgNPs led to cell morphological changes and significant increase in neurite length and enhanced the expression of neuronal differentiation markers such as Map-2, β-tubulin III, synaptophysin, neurogenin-1, Gap-43, and Drd-2. Furthermore, we observed an increase in generation of intracellular reactive oxygen species (ROS), activation of several kinases such as ERK and AKT, and downregulation of expression of dual-specificity phosphatases (DUSPs) in AgNPs-exposed SH-SY5Y cells. Our results suggest that AgNPs modulate the intracellular signaling pathways, leading to neuronal differentiation, and could be applied as promising nanomaterials for stem cell research and therapy.
AB - Nano-scale materials are noted for unique properties, distinct from those of their bulk material equivalents. In this study, we prepared spherical silver nanoparticles (AgNPs) with an average size of about 30 nm and tested their potency to induce neuronal differentiation of SH-SY5Y cells. Human neuroblastoma SH-SY5Y cells are considered an ideal in vitro model for studying neurogenesis, as they can be maintained in an undifferentiated state or be induced to differentiate into neuron-like phenotypes in vitro by several differentiation-inducing agents. Treatment of SH-SY5Y cells by biologically synthesized AgNPs led to cell morphological changes and significant increase in neurite length and enhanced the expression of neuronal differentiation markers such as Map-2, β-tubulin III, synaptophysin, neurogenin-1, Gap-43, and Drd-2. Furthermore, we observed an increase in generation of intracellular reactive oxygen species (ROS), activation of several kinases such as ERK and AKT, and downregulation of expression of dual-specificity phosphatases (DUSPs) in AgNPs-exposed SH-SY5Y cells. Our results suggest that AgNPs modulate the intracellular signaling pathways, leading to neuronal differentiation, and could be applied as promising nanomaterials for stem cell research and therapy.
KW - Dual-specificity phosphatases (DUSPs)
KW - Neuroblastoma
KW - Neuronal differentiation
KW - Reactive oxygen species
KW - Silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84903765217&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84903765217&partnerID=8YFLogxK
U2 - 10.1002/biot.201300555
DO - 10.1002/biot.201300555
M3 - Article
C2 - 24827677
AN - SCOPUS:84903765217
SN - 1860-6768
VL - 9
SP - 934
EP - 943
JO - Biotechnology Journal
JF - Biotechnology Journal
IS - 7
ER -