Biological effects of targeted inactivation of hepatocyte growth factor- like protein in mice

Jorge A. Bezerra, Terri L. Carrick, Jay L. Degen, David Witte, Sandra J.F. Degen

Research output: Contribution to journalArticlepeer-review

67 Scopus citations


Hepatocyte growth factor-like protein (HGFL) is a liver-derived serum glycoprotein involved in cell proliferation and differentiation, and is proposed to have a fundamental role in embryogenesis, fertility, hematopoiesis, macrophage activation, and tissue repair. To assess the in vivo effects of total loss of HGFL, we generated mice with targeted disruption of the gene resulting in loss of the protein. Disruption of the HGFL gene allowed for normal embryogenesis, and followed a Mendelian pattern of genetic transmission. Mice homozygous for the targeted allele (HGFL(-/-) mice) are fertile, and grow to adulthood without obvious phenotypic abnormalities in unchallenged animals, except for development of lipid- containing cytoplasmic vacuoles in hepatocytes throughout the liver lobules. These histologic changes are not accompanied by discernible changes in synthetic or excretory hepatic functions. Hematopoiesis appears unaltered, and although macrophage activation is delayed in the absence of HGFL, migration to the peritoneal cavity upon challenge with thioglycollate was similar in HGFL(-/-) and wild-type mice. Challenged with incision to skin, HGFL(-/-) mice display normal would healing. These data demonstrate that HGFL is not essential for embryogenesis, fertility, or wound healing. HGFL- deficient mice will provide a valuable means to assess the role of HGFL in hepatic and systemic responses to inflammatory and infectious stimuli in vivo.

Original languageEnglish (US)
Pages (from-to)1175-1183
Number of pages9
JournalJournal of Clinical Investigation
Issue number5
StatePublished - Mar 1 1998
Externally publishedYes


  • Growth factor
  • HGF
  • HGFL
  • Macrophages
  • MSP

ASJC Scopus subject areas

  • Medicine(all)


Dive into the research topics of 'Biological effects of targeted inactivation of hepatocyte growth factor- like protein in mice'. Together they form a unique fingerprint.

Cite this