TY - JOUR
T1 - Biochemical characterization of two DNA ligases from Deinococcus radiodurans
AU - Le, Donghai
AU - Hua, Xiaoting
AU - Huang, Lifen
AU - Gao, Guanjun
AU - Lu, Huiming
AU - Xu, Zhenjian
AU - Tian, Bing
AU - Hua, Yuejin
PY - 2008/7/1
Y1 - 2008/7/1
N2 - Two genes encoding a NAD+ -dependent DNA ligase (LigA) and an ATP-dependent DNA ligase (LigB) were identified in the genome of the extremely radioresistant bacterium, Deinococcus radiodurans (DR). The recombinant enzymes expressed in Escherichia coli, were purified to homogeneity and characterized. The optimal temperature and pH value of the two DNA ligases were 60 °C and 7.0, respectively. Their optimal concentration of MgCl2 was 5mM. Their half-lifes of heat inactivation at 100 °C were about 3 min and 5 min, respectively. In addition, the results showed that DRLigB displayed higher activity than DRLigA at stick and blunt ended joining of DNA, indicating that DRLigB is a key DNA ligase of D. radiodurans in DNA recombination and double-strand break repair.
AB - Two genes encoding a NAD+ -dependent DNA ligase (LigA) and an ATP-dependent DNA ligase (LigB) were identified in the genome of the extremely radioresistant bacterium, Deinococcus radiodurans (DR). The recombinant enzymes expressed in Escherichia coli, were purified to homogeneity and characterized. The optimal temperature and pH value of the two DNA ligases were 60 °C and 7.0, respectively. Their optimal concentration of MgCl2 was 5mM. Their half-lifes of heat inactivation at 100 °C were about 3 min and 5 min, respectively. In addition, the results showed that DRLigB displayed higher activity than DRLigA at stick and blunt ended joining of DNA, indicating that DRLigB is a key DNA ligase of D. radiodurans in DNA recombination and double-strand break repair.
KW - DNA ligase
KW - DNA recombination
KW - Deinococcus radiodurans
KW - Double-strand break repair
KW - Metal cofactor
KW - Stick and blunt ended DNA ligation
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U2 - 10.2174/092986608784967010
DO - 10.2174/092986608784967010
M3 - Article
C2 - 18680457
AN - SCOPUS:47249160306
SN - 0929-8665
VL - 15
SP - 600
EP - 605
JO - Protein and Peptide Letters
JF - Protein and Peptide Letters
IS - 6
ER -