TY - JOUR
T1 - Automation in flow cytometry
T2 - Guidelines and review of systems
AU - Al-Attar, Ahmad
AU - Kumar, Kirthi R.
AU - Untersee, Dorian
AU - O'Driscoll, Marci
AU - Ventura, Miguel Francoise S.
AU - Lin, Leo
N1 - Publisher Copyright:
© 2023 International Clinical Cytometry Society.
PY - 2023
Y1 - 2023
N2 - Automation in flow cytometry has recently advanced from the partial laboratory automation and robotics islets, to more fully integrated systems. This article reviews three manufacturers' newest sample preparation systems: the Beckman CellMek, the Sysmex PS-10, and the BD FACSDuet. These three instruments are capable of performing many of the manual steps in flow cytometry sample processing (pipetting, staining, lysing, washing, fixing). General description, capabilities, advantages, and disadvantages of each system are compared. Overall, these systems have the potential to become mainstay items in today's busy clinical flow cytometry laboratories, and save a significant amount of hands-on time for laboratory staff.
AB - Automation in flow cytometry has recently advanced from the partial laboratory automation and robotics islets, to more fully integrated systems. This article reviews three manufacturers' newest sample preparation systems: the Beckman CellMek, the Sysmex PS-10, and the BD FACSDuet. These three instruments are capable of performing many of the manual steps in flow cytometry sample processing (pipetting, staining, lysing, washing, fixing). General description, capabilities, advantages, and disadvantages of each system are compared. Overall, these systems have the potential to become mainstay items in today's busy clinical flow cytometry laboratories, and save a significant amount of hands-on time for laboratory staff.
KW - automation
KW - flow cytometry
KW - immune monitoring
KW - immunophenotyping
KW - sample preperation system
KW - standardization
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U2 - 10.1002/cyto.b.22125
DO - 10.1002/cyto.b.22125
M3 - Review article
C2 - 37178358
AN - SCOPUS:85159162571
SN - 1552-4949
JO - Cytometry Part B - Clinical Cytometry
JF - Cytometry Part B - Clinical Cytometry
ER -