TY - JOUR
T1 - Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system
AU - Shen, Zhiyuan
AU - Pardington-Purtymun, Paige E.
AU - Comeaux, Jarmon C.
AU - Moyzis, Robert K.
AU - Chen, David J.
N1 - Funding Information:
This research was supported by the US Department of Energy and NIH Grant CA50519 to D.J.C. and by a director's postdoctoral fellowship from Los Alamos National Laboratory to Z.S. We thank Drs. E. Morton Bradbury, Scott Peterson, and Robert Cary for helpful discussions and a critical reading of the manuscript.
PY - 1996/10/15
Y1 - 1996/10/15
N2 - The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the 'bait' in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53- mediated pathway(s), and ubiquitination interact through human UBE2I.
AB - The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the 'bait' in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53- mediated pathway(s), and ubiquitination interact through human UBE2I.
UR - http://www.scopus.com/inward/record.url?scp=0030588127&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030588127&partnerID=8YFLogxK
U2 - 10.1006/geno.1996.0540
DO - 10.1006/geno.1996.0540
M3 - Article
C2 - 8921390
AN - SCOPUS:0030588127
SN - 0888-7543
VL - 37
SP - 183
EP - 186
JO - Genomics
JF - Genomics
IS - 2
ER -