TY - JOUR
T1 - Association between dietary phosphate intake and skeletal muscle energetics in adults without cardiovascular disease
AU - Giacona, John M.
AU - Afridi, Areeb
AU - Petric, Ursa Bezan
AU - Johnson, Talon
AU - Pastor, Johanne
AU - Ren, Jimin
AU - Sandon, Lona
AU - Malloy, Craig
AU - Pandey, Ambarish
AU - Shah, Amil
AU - Berry, Jarett D.
AU - Moe, Orson W.
AU - Vongpatanasin, Wanpen
N1 - Publisher Copyright:
© 2024 the American Physiological Society.
PY - 2024/4
Y1 - 2024/4
N2 - Highly bioavailable inorganic phosphate (Pi) is present in large quantities in the typical Western diet and represents a large fraction of total phosphate intake. Dietary Pi excess induces exercise intolerance and skeletal muscle mitochondrial dysfunction in normal mice. However, the relevance of this to humans remains unknown. The study was conducted on 13 individuals without a history of cardiopulmonary disease (46% female, 15% Black participants) enrolled in the pilot-phase of the Dallas Heart and Mind Study. Total dietary phosphate was estimated from 24-h dietary recall (ASA24). Muscle ATP synthesis was measured at rest, and phosphocreatinine (PCr) dynamics was measured during plantar flexion exercise using 7-T 31P magnetic resonance (MR) spectroscopy in the calf muscle. Correlation was assessed between dietary phosphate intake normalized to total caloric intake, resting ATP synthesis, and PCr depletion during exercise. Higher dietary phosphate intake was associated with lower resting ATP synthesis (r ¼ –0.62, P ¼ 0.03), and with higher levels of PCr depletion during plantar flexion exercise relative to the resting period (r ¼ –0.72; P ¼ 0.004). These associations remain significant after adjustment for age and estimated glomerular filtration rate (both P < 0.05). High dietary phosphate intake was also associated with lower serum Klotho levels, and Klotho levels are in turn associated with PCr depletion and higher ADP accumulation post exercise. Our study suggests that higher dietary phosphate is associated with reduced skeletal muscle mitochondrial function at rest and exercise in humans providing new insight into potential mechanisms linking the Western diet to impaired energy metabolism.
AB - Highly bioavailable inorganic phosphate (Pi) is present in large quantities in the typical Western diet and represents a large fraction of total phosphate intake. Dietary Pi excess induces exercise intolerance and skeletal muscle mitochondrial dysfunction in normal mice. However, the relevance of this to humans remains unknown. The study was conducted on 13 individuals without a history of cardiopulmonary disease (46% female, 15% Black participants) enrolled in the pilot-phase of the Dallas Heart and Mind Study. Total dietary phosphate was estimated from 24-h dietary recall (ASA24). Muscle ATP synthesis was measured at rest, and phosphocreatinine (PCr) dynamics was measured during plantar flexion exercise using 7-T 31P magnetic resonance (MR) spectroscopy in the calf muscle. Correlation was assessed between dietary phosphate intake normalized to total caloric intake, resting ATP synthesis, and PCr depletion during exercise. Higher dietary phosphate intake was associated with lower resting ATP synthesis (r ¼ –0.62, P ¼ 0.03), and with higher levels of PCr depletion during plantar flexion exercise relative to the resting period (r ¼ –0.72; P ¼ 0.004). These associations remain significant after adjustment for age and estimated glomerular filtration rate (both P < 0.05). High dietary phosphate intake was also associated with lower serum Klotho levels, and Klotho levels are in turn associated with PCr depletion and higher ADP accumulation post exercise. Our study suggests that higher dietary phosphate is associated with reduced skeletal muscle mitochondrial function at rest and exercise in humans providing new insight into potential mechanisms linking the Western diet to impaired energy metabolism.
KW - dietary phosphate
KW - exercise intolerance
KW - mitochondrial function
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U2 - 10.1152/japplphysiol.00818.2023
DO - 10.1152/japplphysiol.00818.2023
M3 - Article
C2 - 38482570
AN - SCOPUS:85190900257
SN - 8750-7587
VL - 136
SP - 1007
EP - 1014
JO - Journal of applied physiology
JF - Journal of applied physiology
IS - 4
ER -