TY - JOUR
T1 - Assay of urinary oxalate
T2 - Six methodologies compared
AU - Zerwekh, J. E.
AU - Drake, E.
AU - Gregory, J.
AU - Griffith, D.
AU - Hofmann, A. F.
AU - Menon, M.
AU - Pak, C. Y.
PY - 1983
Y1 - 1983
N2 - To assess how well results by different methods for urinary oxalate determinations agree with each other in a clinical setting, we compared six different assays: Hodgkinson and Williams, enzymatic, modified Hodgkinson and Williams, gas chromatography, ion chromatography, and 'high-pressure' liquid chromatography. For the entire group of samples, the mean value by each method agreed relatively closely, although the enzymatic procedure produced a somewhat higher value. All six methods had large coefficients of variation within (8-58%) and between (15-88%) assays. In addition, analytical recovery by most assays was more than 100% of the added oxalate. Analytical recovery of 10 μg of oxalate added per milliliter of urine specimen ranged from 86 to 237%; for 20 μg/mL it was 83 to 320%. Thus for the six methods evaluated, no single method appeared to be superior to the others.
AB - To assess how well results by different methods for urinary oxalate determinations agree with each other in a clinical setting, we compared six different assays: Hodgkinson and Williams, enzymatic, modified Hodgkinson and Williams, gas chromatography, ion chromatography, and 'high-pressure' liquid chromatography. For the entire group of samples, the mean value by each method agreed relatively closely, although the enzymatic procedure produced a somewhat higher value. All six methods had large coefficients of variation within (8-58%) and between (15-88%) assays. In addition, analytical recovery by most assays was more than 100% of the added oxalate. Analytical recovery of 10 μg of oxalate added per milliliter of urine specimen ranged from 86 to 237%; for 20 μg/mL it was 83 to 320%. Thus for the six methods evaluated, no single method appeared to be superior to the others.
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U2 - 10.1093/clinchem/29.11.1977
DO - 10.1093/clinchem/29.11.1977
M3 - Article
C2 - 6627639
AN - SCOPUS:0020512125
SN - 1744-165X
VL - 29
SP - 1977
EP - 1980
JO - Unknown Journal
JF - Unknown Journal
IS - 11
ER -