Assay of urinary oxalate: Six methodologies compared

J. E. Zerwekh, E. Drake, J. Gregory, D. Griffith, A. F. Hofmann, M. Menon, C. Y. Pak

Research output: Contribution to journalArticlepeer-review

44 Scopus citations


To assess how well results by different methods for urinary oxalate determinations agree with each other in a clinical setting, we compared six different assays: Hodgkinson and Williams, enzymatic, modified Hodgkinson and Williams, gas chromatography, ion chromatography, and 'high-pressure' liquid chromatography. For the entire group of samples, the mean value by each method agreed relatively closely, although the enzymatic procedure produced a somewhat higher value. All six methods had large coefficients of variation within (8-58%) and between (15-88%) assays. In addition, analytical recovery by most assays was more than 100% of the added oxalate. Analytical recovery of 10 μg of oxalate added per milliliter of urine specimen ranged from 86 to 237%; for 20 μg/mL it was 83 to 320%. Thus for the six methods evaluated, no single method appeared to be superior to the others.

Original languageEnglish (US)
Pages (from-to)1977-1980
Number of pages4
JournalUnknown Journal
Issue number11
StatePublished - 1983

ASJC Scopus subject areas

  • General Medicine


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