Argonaute and TNRC6, partners in RNAi

Samantha T. Johnson; Krystal C. Johnson; David R. Corey

doi:10.1016/B978-0-12-821595-1.00011-7

Argonaute and TNRC6, partners in RNAi

Samantha T. Johnson, Krystal C. Johnson, David R. Corey

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The control of gene expression by RNA interference (RNAi) requires that small RNAs selectively recognize sequences within the transcriptome. Complementary hybridization between the guide strand of small duplex RNAs and their targets is necessary for recognition. Proteins make a critical contribution to target recognition by protecting small RNAs from degradation by enzymes, facilitating efficient recognition, and enhancing discrimination between target sequences and closely related “off-target” sequences. Here, we focus on the Argonaute (AGO) protein family, the factor that binds directly to small RNAs, and the Trinucleotide repeat-containing protein six (TNRC6) family, scaffolding proteins that help organize gene silencing.

Original language	English (US)
Title of host publication	RNA Therapeutics
Subtitle of host publication	The Evolving Landscape of RNA Therapeutics
Publisher	Elsevier
Pages	17-36
Number of pages	20
ISBN (Electronic)	9780128215951
ISBN (Print)	9780128217399
DOIs	https://doi.org/10.1016/B978-0-12-821595-1.00011-7
State	Published - Jan 1 2022
Externally published	Yes

Keywords

AGO protein
Cytoplasmic nuclear separation
eCLIP
Enzyme
Immunoprecipitation
RNA interference
TNRC6 protein

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/B978-0-12-821595-1.00011-7

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abstract = "The control of gene expression by RNA interference (RNAi) requires that small RNAs selectively recognize sequences within the transcriptome. Complementary hybridization between the guide strand of small duplex RNAs and their targets is necessary for recognition. Proteins make a critical contribution to target recognition by protecting small RNAs from degradation by enzymes, facilitating efficient recognition, and enhancing discrimination between target sequences and closely related “off-target” sequences. Here, we focus on the Argonaute (AGO) protein family, the factor that binds directly to small RNAs, and the Trinucleotide repeat-containing protein six (TNRC6) family, scaffolding proteins that help organize gene silencing.",

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AU - Johnson, Samantha T.

AU - Johnson, Krystal C.

AU - Corey, David R.

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N2 - The control of gene expression by RNA interference (RNAi) requires that small RNAs selectively recognize sequences within the transcriptome. Complementary hybridization between the guide strand of small duplex RNAs and their targets is necessary for recognition. Proteins make a critical contribution to target recognition by protecting small RNAs from degradation by enzymes, facilitating efficient recognition, and enhancing discrimination between target sequences and closely related “off-target” sequences. Here, we focus on the Argonaute (AGO) protein family, the factor that binds directly to small RNAs, and the Trinucleotide repeat-containing protein six (TNRC6) family, scaffolding proteins that help organize gene silencing.

AB - The control of gene expression by RNA interference (RNAi) requires that small RNAs selectively recognize sequences within the transcriptome. Complementary hybridization between the guide strand of small duplex RNAs and their targets is necessary for recognition. Proteins make a critical contribution to target recognition by protecting small RNAs from degradation by enzymes, facilitating efficient recognition, and enhancing discrimination between target sequences and closely related “off-target” sequences. Here, we focus on the Argonaute (AGO) protein family, the factor that binds directly to small RNAs, and the Trinucleotide repeat-containing protein six (TNRC6) family, scaffolding proteins that help organize gene silencing.

KW - AGO protein

KW - Cytoplasmic nuclear separation

KW - eCLIP

KW - Enzyme

KW - Immunoprecipitation

KW - RNA interference

KW - TNRC6 protein

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BT - RNA Therapeutics

PB - Elsevier

ER -

Argonaute and TNRC6, partners in RNAi

Abstract

Keywords

ASJC Scopus subject areas

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