Antisense-Mediated Transcript Knockdown Triggers Premature Transcription Termination

Jong Sun Lee, Joshua T. Mendell

Research output: Contribution to journalArticlepeer-review

78 Scopus citations


Antisense oligonucleotide (ASO)-mediated transcript knockdown is commonly used to interrogate the function of long noncoding RNAs (lncRNAs). Here, Lee and Mendell demonstrate that ASOs induce cleavage of nascent transcripts and thereby trigger premature transcription termination. Thus, these reagents cannot be used to establish an RNA-mediated function for a lncRNA locus.

Original languageEnglish (US)
Pages (from-to)1044-1054.e3
JournalMolecular cell
Issue number5
StatePublished - Mar 5 2020


  • ASO
  • XRN2
  • antisense oligonucleotide
  • lncRNA
  • long noncoding RNA
  • torpedo mechanism
  • transcription termination

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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