@article{75ab3c0a25de4eac845b592473add297,
title = "Antisense-Mediated Transcript Knockdown Triggers Premature Transcription Termination",
abstract = "Antisense oligonucleotide (ASO)-mediated transcript knockdown is commonly used to interrogate the function of long noncoding RNAs (lncRNAs). Here, Lee and Mendell demonstrate that ASOs induce cleavage of nascent transcripts and thereby trigger premature transcription termination. Thus, these reagents cannot be used to establish an RNA-mediated function for a lncRNA locus.",
keywords = "ASO, XRN2, antisense oligonucleotide, lncRNA, long noncoding RNA, torpedo mechanism, transcription termination",
author = "Lee, {Jong Sun} and Mendell, {Joshua T.}",
note = "Funding Information: We thank Albert Mo for technical assistance and Rebecca Burgess and members of the Mendell laboratory for helpful discussions and critical reading of the manuscript. This work was supported by grants from CPRIT ( RP160249 to J.T.M.), the NIH ( R35CA197311 , P30CA142543 , and P50CA196516 to J.T.M.), and the Welch Foundation ( I-1961-20180324 to J.T.M.). J.T.M. is an investigator of the Howard Hughes Medical Institute . Funding Information: We thank Albert Mo for technical assistance and Rebecca Burgess and members of the Mendell laboratory for helpful discussions and critical reading of the manuscript. This work was supported by grants from CPRIT (RP160249 to J.T.M.), the NIH (R35CA197311, P30CA142543, and P50CA196516 to J.T.M.), and the Welch Foundation (I-1961-20180324 to J.T.M.). J.T.M. is an investigator of the Howard Hughes Medical Institute. J.-S.L. and J.T.M. designed the experiments and interpreted the results. J.-S.L. performed all experiments. J.-S.L. and J.T.M. wrote the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2020",
month = mar,
day = "5",
doi = "10.1016/j.molcel.2019.12.011",
language = "English (US)",
volume = "77",
pages = "1044--1054.e3",
journal = "Molecular cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "5",
}