TY - JOUR
T1 - Ancestral hemoglobins in Archaea
AU - Freitas, Tracey Allen K
AU - Hou, Shaobin
AU - Dioum, Elhadji M.
AU - Saito, Jennifer A.
AU - Newhouse, James
AU - Gonzalez, Gonzalo
AU - Gilles-Gonzalez, Marie Alda
AU - Alam, Maqsudul
PY - 2004/4/27
Y1 - 2004/4/27
N2 - Hemoglobins are ubiquitous in Eukarya and Bacteria but, until now, have not been found in Archaea. A phylogenetic analysis of the recently revealed microbial family of globin-coupled heme-based sensors suggests that these sensors descended from an ancient globin-only progenitor, or a protoglobin (Pgb). Here, we report the discovery and characterization of two Pgbs from the Archaea: ApPgb from the obligately aerobic hyperthermophile Aeropyrum pernix, and MaPgb from the strictly anaerobic methanogen Methanosarcina acetivorans. Both ApPgb and MaPgb bind molecular oxygen, nitric oxide, and carbon monoxide by means of a heme moiety that is coordinated to the protein through the F8 histidine (histidine 120). We postulate that these archaeal globins are the ancestors of contemporary hemoglobins.
AB - Hemoglobins are ubiquitous in Eukarya and Bacteria but, until now, have not been found in Archaea. A phylogenetic analysis of the recently revealed microbial family of globin-coupled heme-based sensors suggests that these sensors descended from an ancient globin-only progenitor, or a protoglobin (Pgb). Here, we report the discovery and characterization of two Pgbs from the Archaea: ApPgb from the obligately aerobic hyperthermophile Aeropyrum pernix, and MaPgb from the strictly anaerobic methanogen Methanosarcina acetivorans. Both ApPgb and MaPgb bind molecular oxygen, nitric oxide, and carbon monoxide by means of a heme moiety that is coordinated to the protein through the F8 histidine (histidine 120). We postulate that these archaeal globins are the ancestors of contemporary hemoglobins.
KW - Globin-coupled sensor
KW - Myoglobin
KW - NO sensor
KW - Oxygen sensor
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U2 - 10.1073/pnas.0308657101
DO - 10.1073/pnas.0308657101
M3 - Article
C2 - 15096613
AN - SCOPUS:2342526669
SN - 0027-8424
VL - 101
SP - 6675
EP - 6680
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 17
ER -