An Optogenetic Study of the Electrophysiological Properties of Hippocampal Neurons in PS1-M146V Transgenic Mice (a model of Alzheimer’s disease)

A. I. Erofeev; O. A. Zakharova; S. G. Terekhin; P. V. Plotnikova; I. B. Bezprozvanny; O. L. Vlasova

doi:10.1007/s11055-019-00715-1

An Optogenetic Study of the Electrophysiological Properties of Hippocampal Neurons in PS1-M146V Transgenic Mice (a model of Alzheimer’s disease)

A. I. Erofeev, O. A. Zakharova, S. G. Terekhin, P. V. Plotnikova, I. B. Bezprozvanny, O. L. Vlasova

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1 Scopus citations

Abstract

We report here a comparative (using chemical, electrical, and optical stimulation) study of the electrophysiological properties of cultured hippocampal neurons from transgenic animals expressing a mutant presenilin 1 protein. All three types of stimulation were found to elicit the following differences from the wild type: with overall more active electrophysiological behavior (especially at the beginning of stimulation), mutant cells differed from wild-type neurons by not maintaining consistent activity over time. However, only optogenetic stimulation increased the absolute number of action potentials and decreased their amplitude as compared with the corresponding values in wild-type neurons. The molecular-cellular mechanisms of this effect are suitable for further studies, both basic and applied (to identify regimes for controlling the electrical activity of neurons).

Original language	English (US)
Pages (from-to)	199-207
Number of pages	9
Journal	Neuroscience and Behavioral Physiology
Volume	49
Issue number	2
DOIs	https://doi.org/10.1007/s11055-019-00715-1
State	Published - Feb 15 2019
Externally published	Yes

Keywords

Alzheimer’s disease
hippocampal neurons
optogenetics

ASJC Scopus subject areas

Neuroscience(all)

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10.1007/s11055-019-00715-1

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title = "An Optogenetic Study of the Electrophysiological Properties of Hippocampal Neurons in PS1-M146V Transgenic Mice (a model of Alzheimer{\textquoteright}s disease)",

abstract = "We report here a comparative (using chemical, electrical, and optical stimulation) study of the electrophysiological properties of cultured hippocampal neurons from transgenic animals expressing a mutant presenilin 1 protein. All three types of stimulation were found to elicit the following differences from the wild type: with overall more active electrophysiological behavior (especially at the beginning of stimulation), mutant cells differed from wild-type neurons by not maintaining consistent activity over time. However, only optogenetic stimulation increased the absolute number of action potentials and decreased their amplitude as compared with the corresponding values in wild-type neurons. The molecular-cellular mechanisms of this effect are suitable for further studies, both basic and applied (to identify regimes for controlling the electrical activity of neurons).",

keywords = "Alzheimer{\textquoteright}s disease, hippocampal neurons, optogenetics",

author = "Erofeev, {A. I.} and Zakharova, {O. A.} and Terekhin, {S. G.} and Plotnikova, {P. V.} and Bezprozvanny, {I. B.} and Vlasova, {O. L.}",

note = "Funding Information: This work was supported by the Russian Science Foundation (Grant No. 14-25-00024-P; work corresponding to Figs. 2–4), the Russian Federation Ministry of Education and Science in the framework of the State Assignment No. 17.991.2017/4.6 (work corresponding to Fig. 1).",

year = "2019",

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doi = "10.1007/s11055-019-00715-1",

language = "English (US)",

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T1 - An Optogenetic Study of the Electrophysiological Properties of Hippocampal Neurons in PS1-M146V Transgenic Mice (a model of Alzheimer’s disease)

AU - Erofeev, A. I.

AU - Zakharova, O. A.

AU - Terekhin, S. G.

AU - Plotnikova, P. V.

AU - Bezprozvanny, I. B.

AU - Vlasova, O. L.

N1 - Funding Information: This work was supported by the Russian Science Foundation (Grant No. 14-25-00024-P; work corresponding to Figs. 2–4), the Russian Federation Ministry of Education and Science in the framework of the State Assignment No. 17.991.2017/4.6 (work corresponding to Fig. 1).

PY - 2019/2/15

Y1 - 2019/2/15

N2 - We report here a comparative (using chemical, electrical, and optical stimulation) study of the electrophysiological properties of cultured hippocampal neurons from transgenic animals expressing a mutant presenilin 1 protein. All three types of stimulation were found to elicit the following differences from the wild type: with overall more active electrophysiological behavior (especially at the beginning of stimulation), mutant cells differed from wild-type neurons by not maintaining consistent activity over time. However, only optogenetic stimulation increased the absolute number of action potentials and decreased their amplitude as compared with the corresponding values in wild-type neurons. The molecular-cellular mechanisms of this effect are suitable for further studies, both basic and applied (to identify regimes for controlling the electrical activity of neurons).

AB - We report here a comparative (using chemical, electrical, and optical stimulation) study of the electrophysiological properties of cultured hippocampal neurons from transgenic animals expressing a mutant presenilin 1 protein. All three types of stimulation were found to elicit the following differences from the wild type: with overall more active electrophysiological behavior (especially at the beginning of stimulation), mutant cells differed from wild-type neurons by not maintaining consistent activity over time. However, only optogenetic stimulation increased the absolute number of action potentials and decreased their amplitude as compared with the corresponding values in wild-type neurons. The molecular-cellular mechanisms of this effect are suitable for further studies, both basic and applied (to identify regimes for controlling the electrical activity of neurons).

KW - Alzheimer’s disease

KW - hippocampal neurons

KW - optogenetics

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An Optogenetic Study of the Electrophysiological Properties of Hippocampal Neurons in PS1-M146V Transgenic Mice (a model of Alzheimer’s disease)

Abstract

Keywords

ASJC Scopus subject areas

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