TY - JOUR
T1 - An essential role for mef2c in the cortical response to loss of sleep in mice
AU - Bjorness, Theresa E.
AU - Kulkarni, Ashwinikumar
AU - Rybalchenko, Volodymyr
AU - Suzuki, Ayako
AU - Bridges, Catherine
AU - Harrington, Adam J.
AU - Cowan, Christopher W.
AU - Takahashi, Joseph S.
AU - Konopka, Genevieve
AU - Greene, Robert W.
N1 - Publisher Copyright:
© 2020, eLife Sciences Publications Ltd. All rights reserved.
PY - 2020/8
Y1 - 2020/8
N2 - Neuronal activity and gene expression in response to the loss of sleep can provide a window into the enigma of sleep function. Sleep loss is associated with brain differential gene expression, an increase in pyramidal cell mEPSC frequency and amplitude, and a characteristic rebound and resolution of slow wave sleep-slow wave activity (SWS-SWA). However, the molecular mechanism(s) mediating the sleep loss response are not well understood. We show that sleeploss regulates MEF2C phosphorylation, a key mechanism regulating MEF2C transcriptional activity, and that MEF2C function in postnatal excitatory forebrain neurons is required for the biological events in response to sleep loss in C57BL/6J mice. These include altered gene expression, the increase and recovery of synaptic strength, and the rebound and resolution of SWS-SWA, which implicate MEF2C as an essential regulator of sleep function. One Sentence Summary: MEF2C is critical to the response to sleep loss.
AB - Neuronal activity and gene expression in response to the loss of sleep can provide a window into the enigma of sleep function. Sleep loss is associated with brain differential gene expression, an increase in pyramidal cell mEPSC frequency and amplitude, and a characteristic rebound and resolution of slow wave sleep-slow wave activity (SWS-SWA). However, the molecular mechanism(s) mediating the sleep loss response are not well understood. We show that sleeploss regulates MEF2C phosphorylation, a key mechanism regulating MEF2C transcriptional activity, and that MEF2C function in postnatal excitatory forebrain neurons is required for the biological events in response to sleep loss in C57BL/6J mice. These include altered gene expression, the increase and recovery of synaptic strength, and the rebound and resolution of SWS-SWA, which implicate MEF2C as an essential regulator of sleep function. One Sentence Summary: MEF2C is critical to the response to sleep loss.
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U2 - 10.7554/ELIFE.58331
DO - 10.7554/ELIFE.58331
M3 - Article
C2 - 32851972
AN - SCOPUS:85090636712
SN - 2050-084X
VL - 9
SP - 1
EP - 46
JO - eLife
JF - eLife
ER -