An automatic whole-slide hyperspectral imaging microscope

Minh Ha Tran, Ofelia Gomez, Baowei Fei

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Whole slide imaging (WSI) is a common step used in histopathology to quickly digitize stained histological slides. Digital whole-slide images not only improve the efficiency of labeling but also open the door for computer-aided diagnosis, specifically machine learning-based methods. Hyperspectral imaging (HSI) is an imaging modality that captures data in various wavelengths, some beyond the range of visible lights. In this study, we developed and implemented an automated microscopy system that can acquire hyperspectral whole slide images (HWSI). The system is robust since it consists of parts that can be swapped and bought from different manufacturers. We used the automated system and built a database of 49 HWSI of thyroid cancer. The automatic whole-slide hyperspectral imaging microscope can have many potential applications in biological and medical areas.

Original languageEnglish (US)
Title of host publicationLabel-free Biomedical Imaging and Sensing (LBIS) 2023
EditorsNatan T. Shaked, Oliver Hayden
PublisherSPIE
ISBN (Electronic)9781510658875
DOIs
StatePublished - 2023
EventLabel-free Biomedical Imaging and Sensing (LBIS) 2023 - San Francisco, United States
Duration: Jan 28 2023Jan 31 2023

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume12391
ISSN (Print)1605-7422

Conference

ConferenceLabel-free Biomedical Imaging and Sensing (LBIS) 2023
Country/TerritoryUnited States
CitySan Francisco
Period1/28/231/31/23

Keywords

  • Hyperspectral imaging
  • automation
  • database
  • deep learning
  • whole-slide imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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