Abstract
Clathrin containing coated vesicles prepared from bovine brain catalyzed ATP-driven proton translocation and a 32Pi-ATP exchange reaction. Both activities were measured in the presence of 5 micrograms of oligomycin/mg of protein which completely inhibited these reactions catalyzed by submitochondrial particles. Analyses performed during the purification procedure demonstrated that the oligomycin-resistant pump was concentrated and highly purified in the fractions containing coated vesicles. Moreover, vesicles precipitated by either monoclonal or polyclonal antibodies against clathrin contained the H+ pump activity. Dicyclohexylcarbodiimide (0.5 mM) and N-ethylmaleimide (1 mM) added to the assay mixture inhibited the pump completely, whereas neither vanadate, sodium azide, efrapeptin, or mitochondrial ATPase inhibitor had an effect.
Original language | English (US) |
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Pages (from-to) | 4059-4062 |
Number of pages | 4 |
Journal | Journal of Biological Chemistry |
Volume | 258 |
Issue number | 7 |
State | Published - Apr 10 1983 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology