Albumin protects cultured cerebellar granule neurons against zinc neurotoxicity

Suizhen Lin; Vincent S. Tagliabracci; Xianming Chen; Yansheng Du

doi:10.1097/01.wnr.0000176523.34594.98

Albumin protects cultured cerebellar granule neurons against zinc neurotoxicity

Suizhen Lin, Vincent S. Tagliabracci, Xianming Chen, Yansheng Du

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

In this study, we have investigated the role of albumin in zinc-induced neurotoxicity of cultured rat cerebellar granule neurons. ZnCl₂ induces cerebellar granule cell death in a time- and concentration-dependent manner and albumin in serum affords a significant protection against zinc-induced neurotoxicity. We further measured intracellular zinc concentrations in the presence or absence of albumin. The results showed that exogenously added ZnCl₂ induced an increase in intracellular zinc concentrations in a concentration-dependent fashion in albumin-free buffer. However, in the presence of albumin, it dramatically decreased ZnCl ₂-induced elevation of intracellular zinc concentrations.These results indicate that albumin protects against zinc-induced neurotoxicity by blocking extracellular zinc uptake by neurons.

Original language	English (US)
Pages (from-to)	1461-1465
Number of pages	5
Journal	NeuroReport
Volume	16
Issue number	13
DOIs	https://doi.org/10.1097/01.wnr.0000176523.34594.98
State	Published - Sep 8 2005

Keywords

Albumin
Neuronal death
Neuroprotection
Zinc

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/01.wnr.0000176523.34594.98

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title = "Albumin protects cultured cerebellar granule neurons against zinc neurotoxicity",

abstract = "In this study, we have investigated the role of albumin in zinc-induced neurotoxicity of cultured rat cerebellar granule neurons. ZnCl2 induces cerebellar granule cell death in a time- and concentration-dependent manner and albumin in serum affords a significant protection against zinc-induced neurotoxicity. We further measured intracellular zinc concentrations in the presence or absence of albumin. The results showed that exogenously added ZnCl2 induced an increase in intracellular zinc concentrations in a concentration-dependent fashion in albumin-free buffer. However, in the presence of albumin, it dramatically decreased ZnCl 2-induced elevation of intracellular zinc concentrations.These results indicate that albumin protects against zinc-induced neurotoxicity by blocking extracellular zinc uptake by neurons.",

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T1 - Albumin protects cultured cerebellar granule neurons against zinc neurotoxicity

AU - Lin, Suizhen

AU - Tagliabracci, Vincent S.

AU - Chen, Xianming

AU - Du, Yansheng

PY - 2005/9/8

Y1 - 2005/9/8

N2 - In this study, we have investigated the role of albumin in zinc-induced neurotoxicity of cultured rat cerebellar granule neurons. ZnCl2 induces cerebellar granule cell death in a time- and concentration-dependent manner and albumin in serum affords a significant protection against zinc-induced neurotoxicity. We further measured intracellular zinc concentrations in the presence or absence of albumin. The results showed that exogenously added ZnCl2 induced an increase in intracellular zinc concentrations in a concentration-dependent fashion in albumin-free buffer. However, in the presence of albumin, it dramatically decreased ZnCl 2-induced elevation of intracellular zinc concentrations.These results indicate that albumin protects against zinc-induced neurotoxicity by blocking extracellular zinc uptake by neurons.

AB - In this study, we have investigated the role of albumin in zinc-induced neurotoxicity of cultured rat cerebellar granule neurons. ZnCl2 induces cerebellar granule cell death in a time- and concentration-dependent manner and albumin in serum affords a significant protection against zinc-induced neurotoxicity. We further measured intracellular zinc concentrations in the presence or absence of albumin. The results showed that exogenously added ZnCl2 induced an increase in intracellular zinc concentrations in a concentration-dependent fashion in albumin-free buffer. However, in the presence of albumin, it dramatically decreased ZnCl 2-induced elevation of intracellular zinc concentrations.These results indicate that albumin protects against zinc-induced neurotoxicity by blocking extracellular zinc uptake by neurons.

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KW - Neuroprotection

KW - Zinc

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Albumin protects cultured cerebellar granule neurons against zinc neurotoxicity

Abstract

Keywords

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