TY - JOUR
T1 - Advances in mass spectrometry-based single-cell metabolite analysis
AU - Wang, Bohong
AU - Yao, Ke
AU - Hu, Zeping
N1 - Funding Information:
We thank members of the Hu laboratory for critiquing the manuscript. ZH is supported by grants from the National Natural Science Foundation of China ( 32150024 and 92057209 ), Agilent Applications and Core Technology University Research (ACT-UR) grant (Research Gifts 4483 & 4214 ), Tsinghua-Peking Joint Center for Life Sciences, Beijing Frontier Research Center for Biological Structure, and Boehringer-Ingelheim (China) . We appreciate many other excellent publications that have contributed to the advance of single-cell metabolomics and its application in various biological areas. Due to the space limitation, we regret that we were unable to include all the relevant work in this review.
Publisher Copyright:
© 2023
PY - 2023/6
Y1 - 2023/6
N2 - Cells with near-identical genomic DNA sequences may exhibit differing phenotypes and carry out distinct physiological functions. Such cell-to-cell variability widely exists in different organisms but is poorly understood. Providing the most sensitive reflection of cellular phenotype, metabolomics at the single-cell level may enable us to uncover the hidden world beneath such cellular heterogeneity. However, the limited analyte amount and rapid turnover rate of single-cell metabolome impose a huge challenge to the metabolomic techniques. Over the past decade, the advances in mass spectrometry have played a crucial role in driving the progress of single-cell metabolite profiling. Herein, in this article we focus on the latest advances in mass spectrometry-based single-cell metabolite analysis, highlighting the technical innovations and their applications in biological studies.
AB - Cells with near-identical genomic DNA sequences may exhibit differing phenotypes and carry out distinct physiological functions. Such cell-to-cell variability widely exists in different organisms but is poorly understood. Providing the most sensitive reflection of cellular phenotype, metabolomics at the single-cell level may enable us to uncover the hidden world beneath such cellular heterogeneity. However, the limited analyte amount and rapid turnover rate of single-cell metabolome impose a huge challenge to the metabolomic techniques. Over the past decade, the advances in mass spectrometry have played a crucial role in driving the progress of single-cell metabolite profiling. Herein, in this article we focus on the latest advances in mass spectrometry-based single-cell metabolite analysis, highlighting the technical innovations and their applications in biological studies.
KW - Cellular heterogeneity
KW - Mass spectrometry
KW - Single-cell metabolite analysis
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U2 - 10.1016/j.trac.2023.117075
DO - 10.1016/j.trac.2023.117075
M3 - Review article
AN - SCOPUS:85156143409
SN - 0165-9936
VL - 163
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
M1 - 117075
ER -