TY - JOUR
T1 - Acute megakaryoblastic leukemia associated with trisomy 21 demonstrates a distinct immunophenotype
AU - Wang, Linlin
AU - Peters, John M.
AU - Fuda, Franklin
AU - Li, Long
AU - Karandikar, Nitin J.
AU - Koduru, Prasad
AU - Wang, Huan You
AU - Chen, Weina
N1 - Publisher Copyright:
© 2014 International Clinical Cytometry Society.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Background Acute megakaryoblastic leukemia (AMKL) is a rare form of acute myeloid leukemia that encompasses three distinct subgroups: children with Down syndrome (DS-AMKL), children without DS (non-DS-AMKL), and adults (adult-AMKL). We hypothesize that the biological differences in the subtypes of AMKL may be reflected in distinct immunophenotypic (IP) features. Our aims were to employ mutiparameter flow cytometry to establish a comprehensive IP spectrum of AMKL in multiple demographic groups and to determine whether the subtypes of AMKL demonstrate distinct immunophenotypes. Methods Thirty-seven AMKL cases were retrieved and subdivided into these three groups. Multiparameter flow cytometry was used to determine and compare the immunophenotypes. Available clinical, cytogenetic, and morphologic data were reviewed to confirm diagnoses. Results While there is commonality in the expression pattern for a wide range of myelomonocytic markers in these three subtypes of AMKL, blasts in DS-AMKL were more likely to express CD7 and CD11b than those in non-DS-AMKL and adult-AMKL. Furthermore, blasts in DS-AMKL were more likely to express CD13, CD33, and CD36 than non-DS pediatric AMKL, and showed greater CD56 expression compared with adult AMKL. Conclusions These results indicate that DS-AMKL is related to but immunophenotypically distinct from non-DS-AMKL and adult-AMKL. This distinct immunophenotypic pattern, co-expression of CD7 and CD11b, has practical implications for characterization of AMKL.
AB - Background Acute megakaryoblastic leukemia (AMKL) is a rare form of acute myeloid leukemia that encompasses three distinct subgroups: children with Down syndrome (DS-AMKL), children without DS (non-DS-AMKL), and adults (adult-AMKL). We hypothesize that the biological differences in the subtypes of AMKL may be reflected in distinct immunophenotypic (IP) features. Our aims were to employ mutiparameter flow cytometry to establish a comprehensive IP spectrum of AMKL in multiple demographic groups and to determine whether the subtypes of AMKL demonstrate distinct immunophenotypes. Methods Thirty-seven AMKL cases were retrieved and subdivided into these three groups. Multiparameter flow cytometry was used to determine and compare the immunophenotypes. Available clinical, cytogenetic, and morphologic data were reviewed to confirm diagnoses. Results While there is commonality in the expression pattern for a wide range of myelomonocytic markers in these three subtypes of AMKL, blasts in DS-AMKL were more likely to express CD7 and CD11b than those in non-DS-AMKL and adult-AMKL. Furthermore, blasts in DS-AMKL were more likely to express CD13, CD33, and CD36 than non-DS pediatric AMKL, and showed greater CD56 expression compared with adult AMKL. Conclusions These results indicate that DS-AMKL is related to but immunophenotypically distinct from non-DS-AMKL and adult-AMKL. This distinct immunophenotypic pattern, co-expression of CD7 and CD11b, has practical implications for characterization of AMKL.
KW - Down syndrome
KW - acute megakaryoblastic leukemia
KW - flow cytometry
KW - immunophenotype
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U2 - 10.1002/cyto.b.21198
DO - 10.1002/cyto.b.21198
M3 - Article
C2 - 25361478
AN - SCOPUS:84932113811
SN - 1552-4949
VL - 88
SP - 244
EP - 252
JO - Cytometry Part B - Clinical Cytometry
JF - Cytometry Part B - Clinical Cytometry
IS - 4
ER -