TY - JOUR
T1 - A variant of DNA polymerase β is not cancer specific
AU - Bu, Dawei
AU - Cler, Leslie R.
AU - Lewis, Cheryl M.
AU - Euhus, David M.
PY - 2004/11
Y1 - 2004/11
N2 - DNA Polymerase β (Pol β) carries out base-excision repair (BER) required for DNA maintenance, replication, and recombination in eukaryotic cells. A variant characterized by a deletion of exon 11, an 87-bp region in the catalytic domain (pol βΔ208-236), was previously described as a possible cause of genomic instability in cancer. The variant form was hypothesized to act in a dominant negative fashion, due to the fact that the variant inhibits the gap filling and DNA binding activities of the wild-type pol β protein. DNA polymerase β transcripts were analyzed in 8 breast cancer cell lines, snap-frozen benign breast tissues from 10 women, and lymphocytes from 10 normal controls, using reverse-transcription polymerase chain reaction (RT-PCR) and three separate primer pairs. The exon 10-12 splice site (variant) was identified using a primer designed to span the spliced exons and by sequencing RT-PCR products that included exon 10, exon 11 (if present), and exon 12. In all of the samples tested, we found both the wild-type and exon 11 87-bp deleted variant mRNAs expressed. We conclude that expression of the DNA polymerase β variant (pol βΔ208-236) is ubiquitous and not breast cancer specific.
AB - DNA Polymerase β (Pol β) carries out base-excision repair (BER) required for DNA maintenance, replication, and recombination in eukaryotic cells. A variant characterized by a deletion of exon 11, an 87-bp region in the catalytic domain (pol βΔ208-236), was previously described as a possible cause of genomic instability in cancer. The variant form was hypothesized to act in a dominant negative fashion, due to the fact that the variant inhibits the gap filling and DNA binding activities of the wild-type pol β protein. DNA polymerase β transcripts were analyzed in 8 breast cancer cell lines, snap-frozen benign breast tissues from 10 women, and lymphocytes from 10 normal controls, using reverse-transcription polymerase chain reaction (RT-PCR) and three separate primer pairs. The exon 10-12 splice site (variant) was identified using a primer designed to span the spliced exons and by sequencing RT-PCR products that included exon 10, exon 11 (if present), and exon 12. In all of the samples tested, we found both the wild-type and exon 11 87-bp deleted variant mRNAs expressed. We conclude that expression of the DNA polymerase β variant (pol βΔ208-236) is ubiquitous and not breast cancer specific.
KW - Breast cancer
KW - DNA polymerase β
KW - Polymerase β δ208-236
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U2 - 10.1080/08941930490524372
DO - 10.1080/08941930490524372
M3 - Article
C2 - 15764500
AN - SCOPUS:12144280841
SN - 0894-1939
VL - 17
SP - 327
EP - 331
JO - Journal of Investigative Surgery
JF - Journal of Investigative Surgery
IS - 6
ER -